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ICFRE Scientist and technical recruitment Vegetative Propagation & Forest Biotechnology: practice questions

ICFRE Scientist and technical recruitment · India · Indian Council of Forestry Research and Education

Original practice questions written to the published syllabus · not past papers
10worked here, free
24written to this exam in this subject
8syllabus units

What this subject covers

Producing trees without seed and using biotechnology in forestry: cuttings, grafting, layering, tissue culture, and the molecular tools used in tree breeding and timber identification.

In the exams: IFoS Forestry Paper I Section A (methods of propagation, grafting); ICAR AICE-JRF 59 Unit 3 (vegetative propagation, tissue culture, growth substances); ICAR AIEEA-PG Group 09 (forest biotechnology)

Where the 24 questions fall

How our questions for ICFRE Scientist and technical recruitment spread across the units of Vegetative Propagation & Forest Biotechnology. Difficulty: 2 easy, 19 medium, 3 hard.

Clonal nurseries and mini-cutting systems8
DNA barcoding and timber species identification6
Principles of vegetative propagation2
Rooting of cuttings: hormones, mist chambers, juvenility2
Grafting and budding techniques2
Tissue culture: media, micropropagation, somatic embryogenesis2
Air layering and other layering methods1
Genetic transformation of forest trees and its regulation1

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Principles of vegetative propagation · medium

Cuttings from side branches of Araucaria grow horizontally like branches for years. This is:

  1. Cyclophysis
  2. Topophysis
  3. Somaclonal variation
  4. Apical dominance
Answer and working

B. Topophysis

Topophysis is the persistence of traits linked to the propagule's position of origin.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

2 · Rooting of cuttings: hormones, mist chambers, juvenility · medium

Which low-cost system achieves high humidity for rooting tropical tree cuttings without mist?

  1. Non-mist polythene propagator
  2. Open beds in full sun
  3. Hydroponic flood tables in full sun
  4. Dry sand trays
Answer and working

A. Non-mist polythene propagator

Non-mist propagators (Leakey and colleagues) enclose cuttings over a moist base under shade.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

3 · Rooting of cuttings: hormones, mist chambers, juvenility · medium

IBA is often first dissolved in ethanol or alkali, or used as its potassium salt, because:

  1. Ethanol increases auxin activity tenfold
  2. The acid dissolves poorly in water
  3. Water destroys IBA instantly
  4. Potassium is the active rooting agent
Answer and working

B. The acid dissolves poorly in water

IBA has low water solubility; K-IBA is water-soluble.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

4 · Grafting and budding techniques · medium

For a graft to succeed, the most critical anatomical requirement is:

  1. Matching bark colour of the partners
  2. Equal leaf number on scion and stock
  3. Contact between the two piths
  4. Close contact between the cambia
Answer and working

D. Close contact between the cambia

Callus from both cambia bridges the union and forms new vascular tissue.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

5 · Grafting and budding techniques · medium

Why do grafted seed orchard trees flower years earlier than seedlings?

  1. Rootstocks inject flowering hormones daily
  2. Grafting removes all juvenile tissue from roots
  3. The scion keeps the maturity of the crown it came from
  4. Grafted trees receive more fertiliser
Answer and working

C. The scion keeps the maturity of the crown it came from

Mature scions retain reproductive competence.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

6 · Air layering and other layering methods · hard

Why is air layering rarely used to produce timber plantation stock?

  1. Air layers never survive transplanting
  2. It is prohibited under certification rules
  3. It always produces sterile trees
  4. It yields few plants per tree, with mature traits
Answer and working

D. It yields few plants per tree, with mature traits

Low numbers, high labour and crown maturity limit it to special uses.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

7 · Clonal nurseries and mini-cutting systems · medium

Compared with field hedge macro-cuttings, mini-cuttings typically:

  1. Need much more IBA to root
  2. Root faster with less auxin
  3. Root only in winter months
  4. Produce plants without roots
Answer and working

B. Root faster with less auxin

Juvenile, well-fed mini-stumps give cuttings that root quickly and well.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

8 · Clonal nurseries and mini-cutting systems · medium

What is the purpose of the hardening stage before dispatch?

  1. To stimulate new shoots on the stock plant
  2. To induce roots on fresh cuttings
  3. To sterilise the rooting medium
  4. To toughen plants for the field
Answer and working

D. To toughen plants for the field

Hardening lignifies stems and prepares plants for field stress.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

9 · DNA barcoding and timber species identification · hard

For DNA timber identification to stand up in court, which is essential?

  1. Validated methods and custody records
  2. A single sample tested by any laboratory
  3. Matching wood colour to a photograph
  4. Using only plastid barcodes for origin
Answer and working

A. Validated methods and custody records

Legal defensibility needs validation, custody records and vouchered references.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

10 · DNA barcoding and timber species identification · medium

An auditor suspects that timber declared as a plantation species is in fact a protected species. The most direct test of the species claim is:

  1. Wood anatomy or DNA identification against vouchered references
  2. Checking the declared volume against the shipping invoice
  3. Stable isotope testing of the region where the logs grew
  4. Counting growth rings to confirm the plantation's age
Answer and working

A. Wood anatomy or DNA identification against vouchered references

Species claims are tested by identifying the wood itself, by anatomy or DNA, against vouchered reference material. Isotope ratios test geographic origin, not species; volumes and ring counts do not identify species.

Further reading: See the manual sources: Hartmann and Kester; Leakey et al. 1990; George et al.; Xavier et al.; CBOL 2009; FAO 2004

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Read the subject

Vegetative Propagation & Forest BiotechnologyThe full study manual, free ICFRE Scientist and technical recruitmentFormat, sections and how it has changed

Vegetative Propagation & Forest Biotechnology for other exams

ICAR AICE-JRF/SRF · IndiaNational Postgraduate Entrance Exam · China

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