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GATE Functional Foods, Nutraceuticals & Health Claims: practice questions

GATE — Life Sciences (XL), Section U Food Technology · India · IITs and IISc on rotation (2026: IIT Guwahati)

Original practice questions written to the published syllabus · not past papers
10worked here, free
41written to this exam in this subject
7syllabus units

What this subject covers

Foods and ingredients with health benefits beyond basic nutrition: bioactive compounds, probiotics and prebiotics, dietary fibre, omega-3 fatty acids, plant sterols and polyphenols, foods for special dietary uses, the evidence needed for health claims and how claims are regulated in different countries.

In the exams: GATE XE-G Section 1 (nutraceuticals); ICAR AICE 35.a Unit 8 (nutraceutical and functional foods) and 37 Unit 6

Where the 41 questions fall

How our questions for GATE spread across the units of Functional Foods, Nutraceuticals & Health Claims. Difficulty: 10 easy, 29 medium, 2 hard.

Dietary fibre and the gut microbiome7
Omega-3 fatty acids and structured lipids7
Bioactive compounds: polyphenols, carotenoids, phytosterols and peptides6
Probiotics, prebiotics, synbiotics and postbiotics6
Delivery and stability of bioactives6
Bioactive peptides and protein hydrolysates5
Definitions: functional foods, nutraceuticals and dietary supplements4

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Definitions: functional foods, nutraceuticals and dietary supplements · medium

In the United States, dietary supplements are defined by the

  1. Nutrition Labeling and Education Act
  2. Dietary Supplement Health and Education Act
  3. Federal Meat Inspection Act and its rules
  4. Food Safety Modernization Act, and no other law
Answer and working

B. Dietary Supplement Health and Education Act

DSHEA (1994) defines dietary supplements. The NLEA governs nutrition labelling and health claims, the meat act covers meat inspection, and FSMA covers preventive controls.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

2 · Bioactive compounds: polyphenols, carotenoids, phytosterols and peptides · easy

Genistein and daidzein, found in soy, belong to the class of

  1. Anthocyanins
  2. Carotenoids
  3. Isoflavones
  4. Glucosinolates
Answer and working

C. Isoflavones

They are isoflavones, with weak oestrogen-like activity. Anthocyanins are berry pigments, carotenoids are fat-soluble pigments, and glucosinolates come from cruciferous vegetables.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

3 · Dietary fibre and the gut microbiome · easy

Under the Codex definition, dietary fibre consists of carbohydrate polymers that are

  1. Digested completely to glucose in the small intestine
  2. Found only in the outer bran layers of cereals
  3. Soluble in cold water but not in hot water
  4. Not digested in the human small intestine
Answer and working

D. Not digested in the human small intestine

Codex fibre is carbohydrate polymers (10 or more units, with 3–9 at national discretion) not hydrolysed by the endogenous enzymes of the small intestine. Fibre is not digested to glucose, occurs in many foods and includes insoluble forms.

Further reading: Codex Alimentarius, Guidelines on Nutrition Labelling (dietary fibre definition)

4 · Dietary fibre and the gut microbiome · medium

Starch that has gelatinised and then retrograded on cooling, as in cooled cooked potatoes, is classed as

  1. RS1
  2. RS2
  3. RS3
  4. RS4
Answer and working

C. RS3

Retrograded starch is RS3. RS1 is physically inaccessible starch, RS2 raw granular starch, and RS4 chemically modified starch.

Further reading: Codex Alimentarius, Guidelines on Nutrition Labelling (dietary fibre definition)

5 · Dietary fibre and the gut microbiome · medium

Psyllium is widely used in gluten-free bread mainly because it

  1. Contains a high level of gluten-forming protein
  2. Forms a gel that replaces gluten's structure
  3. Adds red colour and a crisp crust to the crumb
  4. Raises the starch content of the dough sharply
Answer and working

B. Forms a gel that replaces gluten's structure

Psyllium husk forms a strong viscous gel that holds gas and gives structure in gluten-free dough. It is gluten-free and does not add colour or starch.

Further reading: Codex Alimentarius, Guidelines on Nutrition Labelling (dietary fibre definition)

6 · Omega-3 fatty acids and structured lipids · medium

OPO structured lipids are produced commercially using

  1. Random chemical interesterification only
  2. sn-1,3-specific lipases
  3. Hydrogenation with nickel catalyst
  4. Fractional crystallisation of fish oil
Answer and working

B. sn-1,3-specific lipases

sn-1,3-specific lipases exchange fatty acids at the outer positions while keeping palmitic acid at sn-2. Random interesterification would scramble positions; hydrogenation and fractionation do not create OPO.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

7 · Bioactive peptides and protein hydrolysates · easy

The lactotripeptides VPP and IPP, formed in milk fermented by Lactobacillus helveticus, inhibit

  1. Angiotensin-converting enzyme
  2. Pancreatic lipase
  3. Salivary amylase
  4. Lactase
Answer and working

A. Angiotensin-converting enzyme

VPP and IPP inhibit ACE in vitro, which is relevant to blood pressure regulation. They do not target lipase, amylase or lactase.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

8 · Bioactive peptides and protein hydrolysates · medium

Bitterness in protein hydrolysates comes mainly from

  1. Free sugars released by hydrolysis of the protein
  2. Peptides with exposed hydrophobic residues
  3. Minerals added during neutralisation
  4. Oxidised fat from the protein source
Answer and working

B. Peptides with exposed hydrophobic residues

Hydrolysis exposes hydrophobic amino acids such as leucine and proline at peptide ends, which taste bitter. Sugars, salts and fat oxidation are not the main cause.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

9 · Delivery and stability of bioactives · hard

A bioactive degrades by first-order kinetics with k = 0.02 per day. The fraction remaining after 60 days is about

  1. 30%
  2. 70%
  3. 98.8%
  4. 50%
Answer and working

A. 30%

Retention = exp(−0.02 × 60) = exp(−1.2) = 0.301, about 30%. 70% is the fraction lost; 98.8% subtracts 1.2 percentage points from 100; 50% assumes 60 days is one half-life, but the half-life is ln 2 ÷ 0.02 = 35 days.

Further reading: Brodkorb et al., INFOGEST protocol, Nature Protocols

10 · Delivery and stability of bioactives · medium

INFOGEST is

  1. A global database of food composition and nutrient values
  2. A plate-count method for counting probiotics in foods
  3. A standardised in vitro digestion protocol
  4. An international regulation on nutrition and health claims
Answer and working

C. A standardised in vitro digestion protocol

INFOGEST is an international consensus protocol simulating oral, gastric and intestinal digestion in vitro. It is not a composition database, a counting method or a regulation.

Further reading: Brodkorb et al., INFOGEST protocol, Nature Protocols

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Read the subject

Functional Foods, Nutraceuticals & Health ClaimsThe full study manual, free GATEFormat, sections and how it has changed

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Functional Foods, Nutraceuticals & Health Claims for other exams

ICAR AICE-JRF/SRF · IndiaGATE · India

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