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ICAR AICE-JRF/SRF Functional Foods, Nutraceuticals & Health Claims: practice questions

ICAR AICE-JRF/SRF (PhD) — Food Processing Technology, Food Safety & Quality, Processing and Food Engineering · India · ICAR through the National Testing Agency

Original practice questions written to the published syllabus · not past papers
10worked here, free
88written to this exam in this subject
11syllabus units

What this subject covers

Foods and ingredients with health benefits beyond basic nutrition: bioactive compounds, probiotics and prebiotics, dietary fibre, omega-3 fatty acids, plant sterols and polyphenols, foods for special dietary uses, the evidence needed for health claims and how claims are regulated in different countries.

In the exams: GATE XE-G Section 1 (nutraceuticals); ICAR AICE 35.a Unit 8 (nutraceutical and functional foods) and 37 Unit 6

Where the 88 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Functional Foods, Nutraceuticals & Health Claims. Difficulty: 14 easy, 55 medium, 19 hard.

Probiotics, prebiotics, synbiotics and postbiotics9
Dietary fibre and the gut microbiome9
Definitions: functional foods, nutraceuticals and dietary supplements8
Bioactive compounds: polyphenols, carotenoids, phytosterols and peptides8
Omega-3 fatty acids and structured lipids8
Bioactive peptides and protein hydrolysates8
Delivery and stability of bioactives8
Clinical evidence and substantiation of claims8
Regulation of health claims and nutraceuticals across countries8
Foods for special dietary and medical purposes7
Infant and elderly nutrition products7

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Definitions: functional foods, nutraceuticals and dietary supplements · medium

A food supplement label states that it 'cures arthritis'. Under food law in most jurisdictions this claim is

  1. Allowed if the product is natural
  2. Allowed if the dose is stated
  3. Allowed for products over a year old
  4. Prohibited, as a medicinal claim
Answer and working

D. Prohibited, as a medicinal claim

Claims to treat, cure or prevent disease are reserved for authorised medicines; a food making them is either illegal or treated as an unauthorised medicine. Naturalness, dose or age of the product do not change this.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

2 · Bioactive compounds: polyphenols, carotenoids, phytosterols and peptides · hard

Most dietary polyphenols reach the blood at low concentrations mainly because they

  1. Are destroyed by cooking at any temperature
  2. Are poorly absorbed and heavily metabolised
  3. Bind permanently to dietary fibre in the mouth
  4. Are excreted unchanged in the breath
Answer and working

B. Are poorly absorbed and heavily metabolised

Polyphenols are poorly absorbed in the small intestine and extensively conjugated in the gut wall and liver or degraded by colonic bacteria. Cooking does not destroy all of them, and the other routes are not the reason.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

3 · Dietary fibre and the gut microbiome · hard

A product label states that it 'improves your microbiome'. Most regulators would reject this as a health claim because

  1. Microbes cannot be affected by diet
  2. Fibre is not a permitted ingredient
  3. Microbiome studies are always in animals
  4. No measurable health benefit is specified
Answer and working

D. No measurable health benefit is specified

A change in microbial composition is not itself a defined health benefit; claims must state a specific, measurable benefit shown in humans. Diet does change the microbiota, fibre is permitted, and many human studies exist.

Further reading: Codex Alimentarius, Guidelines on Nutrition Labelling (dietary fibre definition)

4 · Dietary fibre and the gut microbiome · medium

Psyllium is widely used in gluten-free bread mainly because it

  1. Contains a high level of gluten-forming protein
  2. Forms a gel that replaces gluten's structure
  3. Adds red colour and a crisp crust to the crumb
  4. Raises the starch content of the dough sharply
Answer and working

B. Forms a gel that replaces gluten's structure

Psyllium husk forms a strong viscous gel that holds gas and gives structure in gluten-free dough. It is gluten-free and does not add colour or starch.

Further reading: Codex Alimentarius, Guidelines on Nutrition Labelling (dietary fibre definition)

5 · Omega-3 fatty acids and structured lipids · medium

OPO structured lipids are produced commercially using

  1. Random chemical interesterification only
  2. sn-1,3-specific lipases
  3. Hydrogenation with nickel catalyst
  4. Fractional crystallisation of fish oil
Answer and working

B. sn-1,3-specific lipases

sn-1,3-specific lipases exchange fatty acids at the outer positions while keeping palmitic acid at sn-2. Random interesterification would scramble positions; hydrogenation and fractionation do not create OPO.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

6 · Bioactive peptides and protein hydrolysates · easy

The lactotripeptides VPP and IPP, formed in milk fermented by Lactobacillus helveticus, inhibit

  1. Angiotensin-converting enzyme
  2. Pancreatic lipase
  3. Salivary amylase
  4. Lactase
Answer and working

A. Angiotensin-converting enzyme

VPP and IPP inhibit ACE in vitro, which is relevant to blood pressure regulation. They do not target lipase, amylase or lactase.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

7 · Bioactive peptides and protein hydrolysates · medium

Bitterness in protein hydrolysates comes mainly from

  1. Free sugars released by hydrolysis of the protein
  2. Peptides with exposed hydrophobic residues
  3. Minerals added during neutralisation
  4. Oxidised fat from the protein source
Answer and working

B. Peptides with exposed hydrophobic residues

Hydrolysis exposes hydrophobic amino acids such as leucine and proline at peptide ends, which taste bitter. Sugars, salts and fat oxidation are not the main cause.

Further reading: Gibson and Williams (eds.), Functional Foods: Concept to Product

8 · Foods for special dietary and medical purposes · hard

Why are free amino acid formulas for metabolic disorders difficult to make palatable?

  1. Free amino acids taste bitter and unpleasant
  2. They contain too much fat to emulsify in water
  3. They are too sweet for patients
  4. They cannot be dried into powders
Answer and working

A. Free amino acids taste bitter and unpleasant

Many free amino acids are bitter or sulphurous, so these formulas need flavour masking and careful formulation. Fat, sweetness and drying are not the main problem.

Further reading: Codex Alimentarius, CXS 180-1991 and CXS 118-1979

9 · Delivery and stability of bioactives · hard

A bioactive degrades by first-order kinetics with k = 0.02 per day. The fraction remaining after 60 days is about

  1. 30%
  2. 70%
  3. 98.8%
  4. 50%
Answer and working

A. 30%

Retention = exp(−0.02 × 60) = exp(−1.2) = 0.301, about 30%. 70% is the fraction lost; 98.8% subtracts 1.2 percentage points from 100; 50% assumes 60 days is one half-life, but the half-life is ln 2 ÷ 0.02 = 35 days.

Further reading: Brodkorb et al., INFOGEST protocol, Nature Protocols

10 · Delivery and stability of bioactives · medium

INFOGEST is

  1. A global database of food composition and nutrient values
  2. A plate-count method for counting probiotics in foods
  3. A standardised in vitro digestion protocol
  4. An international regulation on nutrition and health claims
Answer and working

C. A standardised in vitro digestion protocol

INFOGEST is an international consensus protocol simulating oral, gastric and intestinal digestion in vitro. It is not a composition database, a counting method or a regulation.

Further reading: Brodkorb et al., INFOGEST protocol, Nature Protocols

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Read the subject

Functional Foods, Nutraceuticals & Health ClaimsThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

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