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ASRB NET Linear Mixed Models, Variance Components & Spatial Analysis of Field Trials: practice questions

ASRB NET - Agricultural Statistics (and Computer Application / Bioinformatics) · India · Agricultural Scientists Recruitment Board (ICAR)

Original practice questions written to the published syllabus · not past papers
10worked here, free
84written to this exam in this subject
10syllabus units

What this subject covers

Models with more than one source of random variation: ANOVA, ML and REML estimation of variance components, BLUE and BLUP, Henderson's mixed-model equations, and spatial models that correct field trials for fertility trends and neighbour correlation.

In the exams: AICE-JRF Ag Stat Unit 4 'random, fixed and mixed effects' (present); AICE-JRF Ag Stat Unit 3 genetic variance (present); PhD course in most programmes

What ASRB NET asks from it

ASRB NET / ARS Agricultural Statistics

Applied mixed-model analysis: REML versus ANOVA estimation, BLUP for selection, spatial analysis of variety trials, choosing covariance structures and software.

Where the 84 questions fall

How our questions for ASRB NET spread across the units of Linear Mixed Models, Variance Components & Spatial Analysis of Field Trials. Difficulty: 22 easy, 40 medium, 22 hard.

Henderson's mixed-model equations; BLUE and BLUP11
Covariance structures for repeated measures10
Maximum likelihood and REML9
Spatial analysis of field trials: nearest-neighbour and AR1 x AR1 models9
Fixed, random and mixed models revisited8
Unbalanced data and Satterthwaite and Kenward-Roger degrees of freedom8
Model selection with likelihood-ratio tests and information criteria8
ANOVA (Henderson's methods) estimation of variance components7
Trend and spline adjustment (e.g. SpATS approach)7
Software: lme4, nlme, ASReml, SAS PROC MIXED7

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Fixed, random and mixed models revisited · easy

In the mixed model y = X beta + Z u + e, with u ~ N(0, G) and e ~ N(0, R), what is the variance of y?

  1. G + R
  2. Z R Z' + G
  3. Z G Z' + R
  4. X G X' + R
Answer and working

C. Z G Z' + R

V(y) = V(Zu) + V(e) = ZGZ' + R, since u and e are independent and X beta is fixed.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

2 · Fixed, random and mixed models revisited · medium

Which model suits germination counts per pot with random blocks?

  1. A linear mixed model on the raw counts, whatever their distribution
  2. An ordinary least squares model with no blocks
  3. A generalised linear mixed model with a suitable link
  4. A fixed-effects ANOVA on block totals
Answer and working

C. A generalised linear mixed model with a suitable link

Counts or proportions with random effects are naturally handled by GLMMs (e.g. binomial or Poisson with a link function).

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

3 · Henderson's mixed-model equations; BLUE and BLUP · medium

In the mixed model, what is beta-hat from the mixed-model equations?

  1. The BLUP of the random effects
  2. The ML estimate of variance
  3. The OLS estimate, ignoring the covariance matrix V
  4. The BLUE of beta, equal to the GLS estimator
Answer and working

D. The BLUE of beta, equal to the GLS estimator

beta-hat solves the GLS problem with V = ZGZ' + R; u-hat is the BLUP.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

4 · Spatial analysis of field trials: nearest-neighbour and AR1 x AR1 models · easy

Why is spatial analysis used in field trials?

  1. Fertility varies smoothly and irregularly, not only between blocks
  2. Blocks are never useful in field trials
  3. It removes the need for replication
  4. Plots far apart are always more alike than adjacent plots in a block
Answer and working

A. Fertility varies smoothly and irregularly, not only between blocks

Neighbouring plots are more alike; spatial models capture trends that blocks miss.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

5 · Trend and spline adjustment (e.g. SpATS approach) · medium

Can a spline surface and an AR1 x AR1 residual be combined in one model?

  1. Only if the trial has a single row
  2. Only when no genotypes or treatments are fitted in the model
  3. No, they are mathematically incompatible
  4. Yes, for large-scale trend plus local correlation
Answer and working

D. Yes, for large-scale trend plus local correlation

Combining them is possible; the cost is complexity and convergence risk.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

6 · Model selection with likelihood-ratio tests and information criteria · hard

Why is the naive chi-square test conservative when testing a variance component equal to zero?

  1. Variance components are always large
  2. REML likelihoods cannot be compared between any two models at all
  3. The null value lies on the boundary of the parameter space
  4. The chi-square has too many df
Answer and working

C. The null value lies on the boundary of the parameter space

On the boundary the statistic follows a 50:50 mixture, so the naive p-value is twice the correct one.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

7 · Model selection with likelihood-ratio tests and information criteria · medium

Comparing AR(1) and Toeplitz residual structures with the same fixed effects, which tool is appropriate?

  1. Bartlett's test on residuals
  2. REML-based AIC or BIC
  3. A t test on the variety means
  4. ML-based F tests on fixed effects
Answer and working

B. REML-based AIC or BIC

Covariance structures with common fixed effects are compared by REML likelihoods and information criteria.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

8 · Model selection with likelihood-ratio tests and information criteria · medium

Why are replicate and block random terms usually kept even if not significant?

  1. They always reduce AIC
  2. They are required by all software
  3. They reflect the randomisation of the design
  4. They are fixed effects in disguise and are tested like treatments
Answer and working

C. They reflect the randomisation of the design

Design terms protect the validity of inference; significance testing is reserved for optional terms.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

9 · Maximum likelihood and REML · easy

What does REML stand for?

  1. Repeated measures linear model
  2. Restricted (residual) maximum likelihood
  3. Random effects minimum loss
  4. Regression estimate of mean level
Answer and working

B. Restricted (residual) maximum likelihood

REML maximises the likelihood of error contrasts.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

10 · Henderson's mixed-model equations; BLUE and BLUP · easy

What does BLUP stand for?

  1. Bayesian likelihood under priors
  2. Best linear unbiased prediction
  3. Balanced linear unit prediction
  4. Block level uniform precision
Answer and working

B. Best linear unbiased prediction

BLUP predicts random effects; BLUE estimates fixed effects.

Further reading: Searle, Casella and McCulloch, Variance Components; Littell et al., SAS for Mixed Models

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Read the subject

Linear Mixed Models, Variance Components & Spatial Analysis of Field TrialsThe full study manual, free ASRB NETFormat, sections and how it has changed

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Linear Mixed Models, Variance Components & Spatial Analysis of Field Trials for other exams

ICAR AICE-JRF/SRF · India

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