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ASRB NET and ARS Lactose, Milk Salts & Vitamins: practice questions

ASRB NET and ARS: dairy disciplines · India · Agricultural Scientists Recruitment Board (ICAR)

Original practice questions written to the published syllabus · not past papers
10worked here, free
29written to this exam in this subject
10syllabus units

What this subject covers

Lactose chemistry and crystallisation, the salt system of milk, trace elements and water-soluble vitamins.

In the exams: ICAR AICE subject 34 Unit 1; ICAR AIEEA-PG group 16 (lactose crystallisation and its significance)

What ASRB NET and ARS asks from it

ASRB NET and ARS — Dairy Chemistry

Recall at Master's depth: alpha and beta lactose properties, the 93.5 degree transition, sucrose ratio, salt concentrations and partition, stabilising salts, trace elements, vitamin contents and stabilities.

Where the 29 questions fall

How our questions for ASRB NET and ARS spread across the units of Lactose, Milk Salts & Vitamins. Difficulty: 20 easy, 5 medium, 4 hard.

Lactose structure, alpha and beta forms and mutarotation5
Lactose solubility and crystallisation4
Maillard browning and its control4
Lactose biosynthesis and intolerance3
Milk salts: calcium, phosphate, citrate and their partition3
Lactose in sweetened condensed milk, powders and ice cream2
Lactose derivatives: lactulose, galacto-oligosaccharides, lactitol, lactobionic acid2
Salt balance and heat stability2
Trace elements in milk2
Water-soluble vitamins and their losses in processing2

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Lactose structure, alpha and beta forms and mutarotation · easy

Compared with sucrose, the sweetness of lactose is roughly:

  1. Ten times as great
  2. Twice as great
  3. Exactly the same
  4. One fifth to one third
Answer and working

D. One fifth to one third

Lactose has low relative sweetness, so milk does not taste sweet despite about 4.7% lactose.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

2 · Lactose solubility and crystallisation · easy

Below about which temperature does lactose crystallise from solution as alpha-lactose monohydrate?

  1. 62.5 degrees Celsius
  2. 93.5 degrees Celsius
  3. 121.0 degrees Celsius
  4. 37.0 degrees Celsius
Answer and working

B. 93.5 degrees Celsius

Below about 93.5 C alpha-monohydrate crystallises; above it, anhydrous beta-lactose.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

3 · Lactose in sweetened condensed milk, powders and ice cream · medium

Why is sweetened condensed milk seeded with finely ground lactose during rapid cooling?

  1. To provide many nuclei so that lactose crystallises as many small crystals
  2. To add sweetness without increasing the sucrose ratio
  3. To raise the water activity so that the product stays fluid
  4. To dissolve the lactose already present and prevent all crystallisation during storage
Answer and working

A. To provide many nuclei so that lactose crystallises as many small crystals

Lactose is supersaturated in SCM, so crystallisation cannot be avoided; seeding at high supersaturation gives many small crystals and a smooth texture.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

4 · Maillard browning and its control · easy

The Maillard reaction in milk occurs mainly between lactose and:

  1. The epsilon-amino group of lysine residues
  2. The carboxyl groups of fatty acids
  3. The phosphate groups of phosphoserine
  4. The sulfhydryl group of cysteine residues
Answer and working

A. The epsilon-amino group of lysine residues

Reducing sugars react with free amino groups, chiefly lysine's epsilon-amino group in proteins.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

5 · Maillard browning and its control · easy

At about what water activity is Maillard browning fastest?

  1. 0.98 to 1.0
  2. Exactly 0.3
  3. 0.1 to 0.2
  4. 0.6 to 0.7
Answer and working

D. 0.6 to 0.7

Browning peaks at intermediate water activity, typical of concentrated and partly dried products.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

6 · Lactose biosynthesis and intolerance · easy

Lactose synthase consists of:

  1. Beta-1,4-galactosyltransferase and alpha-lactalbumin
  2. Lipoprotein lipase and kappa-casein
  3. Glucokinase and serum albumin
  4. Beta-galactosidase and beta-lactoglobulin
Answer and working

A. Beta-1,4-galactosyltransferase and alpha-lactalbumin

Alpha-lactalbumin changes galactosyltransferase specificity so glucose becomes the acceptor.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

7 · Lactose biosynthesis and intolerance · easy

Lactose intolerance is caused by:

  1. Low intestinal lactase activity, leaving lactose undigested
  2. Excess production of lactase in the colon
  3. Inability to metabolise galactose in the liver
  4. An immune reaction against lactose molecules
Answer and working

A. Low intestinal lactase activity, leaving lactose undigested

It is a non-immune malabsorption; galactose metabolism defects cause galactosaemia.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

8 · Milk salts: calcium, phosphate, citrate and their partition · easy

Which ions in milk are almost entirely in the soluble phase?

  1. Zinc and iron
  2. Calcium and citrate
  3. Sodium, potassium and chloride
  4. Calcium, magnesium and inorganic phosphate
Answer and working

C. Sodium, potassium and chloride

Monovalent ions do not bind the micelles; calcium, magnesium and phosphate are partly colloidal.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

9 · Salt balance and heat stability · easy

In the salt balance theory, which ions tend to destabilise milk on heating?

  1. Calcium and magnesium
  2. Potassium and sulphate
  3. Sodium and chloride
  4. Phosphate and citrate
Answer and working

A. Calcium and magnesium

Divalent cations reduce casein charge and bridge micelles; phosphate and citrate bind them and stabilise.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

10 · Trace elements in milk · medium

Which trace element in milk is technologically important mainly as a catalyst of lipid oxidation?

  1. Cobalt
  2. Zinc
  3. Copper
  4. Selenium
Answer and working

C. Copper

Copper, even at fractions of a mg/L, catalyses oxidation; this is why copper fittings were replaced by stainless steel.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

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Read the subject

Lactose, Milk Salts & VitaminsThe full study manual, free ASRB NET and ARSFormat, sections and how it has changed

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Lactose, Milk Salts & Vitamins for other exams

ICAR AIEEA-PG · IndiaICAR AICE-JRF/SRF · India

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