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ICAR AICE-JRF/SRF Lactose, Milk Salts & Vitamins: practice questions

ICAR AICE-JRF/SRF (PhD): Dairy Chemistry, Dairy Microbiology, Dairy Technology, Dairy Engineering · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
80written to this exam in this subject
10syllabus units

What this subject covers

Lactose chemistry and crystallisation, the salt system of milk, trace elements and water-soluble vitamins.

In the exams: ICAR AICE subject 34 Unit 1; ICAR AIEEA-PG group 16 (lactose crystallisation and its significance)

What ICAR AICE-JRF/SRF asks from it

ICAR AICE-JRF/SRF (PhD) — Dairy Chemistry (subject 34, Unit 1)

Lactose chemistry (anomers, mutarotation, solubility, crystallisation, glass transition), Maillard reaction, lactose derivatives, salt partition and salt balance, heat stability, trace elements and vitamins. Questions test mechanisms and their technological consequences, such as why sweetened condensed milk is seeded and why whey powder cakes.

Where the 80 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Lactose, Milk Salts & Vitamins. Difficulty: 16 easy, 41 medium, 23 hard.

Lactose solubility and crystallisation10
Lactose derivatives: lactulose, galacto-oligosaccharides, lactitol, lactobionic acid9
Milk salts: calcium, phosphate, citrate and their partition9
Water-soluble vitamins and their losses in processing9
Lactose in sweetened condensed milk, powders and ice cream8
Maillard browning and its control8
Lactose biosynthesis and intolerance8
Salt balance and heat stability7
Trace elements in milk7
Lactose structure, alpha and beta forms and mutarotation5

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Lactose structure, alpha and beta forms and mutarotation · medium

If the equilibrium ratio of beta to alpha lactose is 1.68, what percentage of the lactose is in the beta form?

  1. 59.5 per cent
  2. 37.3 per cent
  3. 168 per cent
  4. 62.7 per cent
Answer and working

D. 62.7 per cent

Beta fraction = 1.68 / (1 + 1.68) = 0.627 = 62.7%. 37.3 is the alpha share; 59.5 is 1/1.68.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

2 · Lactose in sweetened condensed milk, powders and ice cream · hard

A sucrose ratio of about 62.5 to 64.5 per cent is recommended in sweetened condensed milk because:

  1. Lower values cause lactose to dissolve completely and higher values prevent Maillard browning
  2. Lower values allow microbial growth and higher values risk sucrose crystallisation
  3. Any value above 50 per cent prevents all lactose crystallisation
  4. It is the ratio at which lactose and sucrose have equal solubility
Answer and working

B. Lower values allow microbial growth and higher values risk sucrose crystallisation

The sucrose ratio sets water activity: too low permits yeasts and moulds; too high crystallises sucrose.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

3 · Lactose in sweetened condensed milk, powders and ice cream · hard

Ice cream becomes sandy during storage mainly because:

  1. Fat crystals melt and recrystallise as large beta crystals
  2. Lactose crystallises in the concentrated serum, especially with high MSNF and temperature fluctuations
  3. Ice crystals of more than 1 millimetre form immediately on freezing in the freezer and are felt as grains of sand
  4. Sucrose reacts with milk protein to form brown granules
Answer and working

B. Lactose crystallises in the concentrated serum, especially with high MSNF and temperature fluctuations

Sandiness is lactose crystallisation; coarse iciness is a separate defect from large ice crystals.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

4 · Lactose derivatives: lactulose, galacto-oligosaccharides, lactitol, lactobionic acid · medium

Galacto-oligosaccharides are produced industrially by:

  1. Transgalactosylation with beta-galactosidase at high lactose concentration
  2. Fermentation of lactose to lactic acid
  3. Acid hydrolysis of lactose at low concentration followed by neutralisation
  4. Catalytic hydrogenation of lactose
Answer and working

A. Transgalactosylation with beta-galactosidase at high lactose concentration

At high lactose concentration beta-galactosidase transfers galactose to sugars rather than water, forming GOS.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

5 · Lactose biosynthesis and intolerance · medium

According to the EFSA review, roughly how much lactose in a single dose do most people with lactose malabsorption tolerate?

  1. Up to about 12 grams
  2. No lactose at all
  3. Up to about 100 grams
  4. Up to about 0.1 gram
Answer and working

A. Up to about 12 grams

EFSA (2010) concluded most tolerate up to about 12 g lactose in a single dose, roughly a cup of milk.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

6 · Milk salts: calcium, phosphate, citrate and their partition · medium

Cow milk contains about 1.2 grams per litre of calcium (atomic mass 40.08). What is this in millimoles per litre?

  1. About 30 millimolar
  2. About 300 millimolar
  3. About 48 millimolar
  4. About 3 millimolar
Answer and working

A. About 30 millimolar

1.2/40.08 x 1000 = 29.9 mM. 48 multiplies 1.2 by 40.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

7 · Salt balance and heat stability · hard

In processed cheese, emulsifying salts such as citrates and phosphates work by:

  1. Adding calcium to para-casein so that it forms a firmer and more elastic protein network
  2. Lowering the pH to the isoelectric point of casein
  3. Hydrolysing fat into emulsifying monoglycerides
  4. Sequestering calcium from para-casein so it hydrates and emulsifies fat
Answer and working

D. Sequestering calcium from para-casein so it hydrates and emulsifies fat

Calcium removal disperses para-casein, which then emulsifies the fat.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

8 · Trace elements in milk · medium

Which trace element in milk is technologically important mainly as a catalyst of lipid oxidation?

  1. Cobalt
  2. Zinc
  3. Copper
  4. Selenium
Answer and working

C. Copper

Copper, even at fractions of a mg/L, catalyses oxidation; this is why copper fittings were replaced by stainless steel.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

9 · Trace elements in milk · medium

Selenium content of milk varies mainly with:

  1. The time of day of milking
  2. The selenium content of soils and feed supplements
  3. The storage temperature of the milk
  4. The fat content of the milk
Answer and working

B. The selenium content of soils and feed supplements

Milk selenium reflects soil and dietary selenium.

Further reading: McSweeney and Fox (eds), Advanced Dairy Chemistry Vol. 3: Lactose, Water, Salts and Minor Constituents

10 · Maillard browning and its control · medium

Melanoidins in heated milk products are:

  1. Red pigments formed by bacteria
  2. Colourless Amadori products formed in the early stage of the reaction with lysine
  3. Brown nitrogenous polymers formed in the final stage of the Maillard reaction
  4. Flavour aldehydes formed by lipid oxidation
Answer and working

C. Brown nitrogenous polymers formed in the final stage of the Maillard reaction

Melanoidins are the brown end products of the Maillard reaction.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

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Lactose, Milk Salts & VitaminsThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

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