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Food Safety Officer recruitment Microbiological Quality Assurance & Rapid Methods: practice questions

Food Safety Officer recruitment (state PSCs and FSSAI) · India · State public service commissions; FSSAI for central posts

Original practice questions written to the published syllabus · not past papers
6worked here, free
23written to this exam in this subject
8syllabus units

What this subject covers

How dairy laboratories sample, count, detect and certify: criteria, indicator organisms, rapid methods and lab quality systems.

In the exams: ICAR AICE subject 36 Unit 6 and subject 35b Units 1 and 3; ICAR AIEEA-PG group 16 (enumeration, isolation and identification of pathogens)

Where the 23 questions fall

How our questions for Food Safety Officer recruitment spread across the units of Microbiological Quality Assurance & Rapid Methods. Difficulty: 17 easy, 5 medium, 1 hard.

Indicator organisms: coliforms, Enterobacteriaceae, E. coli4
Biosafety levels and good microbiological practice4
Microbiological criteria and how they are set3
Standard plate count and other enumeration methods3
Laboratory accreditation: ISO/IEC 170253
Detection and isolation of pathogens2
Rapid methods: ATP bioluminescence, PCR, real-time PCR, immunoassays2
Environmental monitoring programmes2

6 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Indicator organisms: coliforms, Enterobacteriaceae, E. coli · easy

Coliforms are defined as Gram-negative non-spore-forming rods that:

  1. Ferment lactose with acid and gas within 48 h at 30–37 °C
  2. Ferment glucose but never lactose
  3. Form heat-resistant spores that survive pasteurisation at 72 °C
  4. Grow only above 55 °C
Answer and working

A. Ferment lactose with acid and gas within 48 h at 30–37 °C

Lactose fermentation with acid and gas is the defining trait; Enterobacteriaceae are defined by glucose fermentation.

Further reading: ICMSF, Microorganisms in Foods 7, 2nd ed.

2 · Indicator organisms: coliforms, Enterobacteriaceae, E. coli · easy

Which indicator is most specific for faecal contamination?

  1. Yeasts and moulds
  2. Escherichia coli
  3. Total coliforms
  4. Total viable count
Answer and working

B. Escherichia coli

E. coli lives mainly in the intestine of warm-blooded animals; many coliforms are environmental.

Further reading: Wehr and Frank (eds), Standard Methods for the Examination of Dairy Products, 17th ed.

3 · Detection and isolation of pathogens · easy

The usual sequence in cultural detection of a pathogen is:

  1. Direct plating only, without enrichment
  2. Pre-enrichment, selective enrichment, plating, confirmation
  3. Confirmation first, then enrichment
  4. Selective plating, then pre-enrichment, then serological confirmation
Answer and working

B. Pre-enrichment, selective enrichment, plating, confirmation

Enrichment resuscitates injured cells and increases target numbers before isolation and confirmation.

Further reading: Wehr and Frank (eds), Standard Methods for the Examination of Dairy Products, 17th ed.

4 · Biosafety levels and good microbiological practice · easy

Salmonella, Listeria monocytogenes and Staphylococcus aureus are handled at:

  1. BSL-4
  2. BSL-2
  3. BSL-3
  4. BSL-1
Answer and working

B. BSL-2

They are risk group 2 agents: disease is possible but treatable and spread is limited.

Further reading: Wehr and Frank (eds), Standard Methods for the Examination of Dairy Products, 17th ed.

5 · Biosafety levels and good microbiological practice · medium

A Class II biological safety cabinet protects:

  1. The worker only
  2. The sample only
  3. Neither the worker nor the sample; it only filters exhaust
  4. The worker, the sample and the environment
Answer and working

D. The worker, the sample and the environment

Inward airflow and HEPA-filtered downflow protect all three; Class I does not protect the sample.

Further reading: Wehr and Frank (eds), Standard Methods for the Examination of Dairy Products, 17th ed.

6 · Laboratory accreditation: ISO/IEC 17025 · easy

In India, testing laboratories are accredited to ISO/IEC 17025 by:

  1. BIS
  2. APEDA
  3. NDDB
  4. NABL
Answer and working

D. NABL

The National Accreditation Board for Testing and Calibration Laboratories is India's ILAC signatory.

Further reading: Wehr and Frank (eds), Standard Methods for the Examination of Dairy Products, 17th ed.

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Read the subject

Microbiological Quality Assurance & Rapid MethodsThe full study manual, free Food Safety Officer recruitmentFormat, sections and how it has changed

More Food Safety Officer recruitment practice

Dairy Analysis & Instrumental MethodsQuality Assurance, Statistical Quality Control & Laboratory Management

Microbiological Quality Assurance & Rapid Methods for other exams

ICAR AICE-JRF/SRF · IndiaFSSAI Food Analyst and Junior Analyst Examinations · IndiaICAR AIEEA-PG · IndiaASRB NET and ARS · India

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