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GATE Food Chemistry II: Pigments, Flavours, Vitamins, Minerals, Enzymes & Browning: practice questions

GATE — Engineering Sciences (XE), Section G Food Technology · India · IITs and IISc on rotation (2026: IIT Guwahati)

Original practice questions written to the published syllabus · not past papers
10worked here, free
120written to this exam in this subject
14syllabus units

What this subject covers

The minor constituents that decide colour, flavour and nutritional value, the enzymes that act in raw and processed food, and the browning reactions — enzymatic and non-enzymatic — that every food technologist must control or exploit.

In the exams: GATE XE-G and XL-U Section 1 (pigments, flavours, enzymes, browning); ICAR AIEEA-PG Code 18 Unit I (food flavours); ICAR AICE 35.a Unit 7 (flavour chemistry technology)

Where the 120 questions fall

How our questions for GATE spread across the units of Food Chemistry II: Pigments, Flavours, Vitamins, Minerals, Enzymes & Browning. Difficulty: 36 easy, 77 medium, 7 hard.

Food enzymes: specificity, kinetics, inhibition and coenzymes11
Pigments: chlorophylls, carotenoids, anthocyanins, betalains, tannins and myoglobin10
Flavour chemistry: terpenes, esters, aldehydes, ketones and sulphur compounds10
Vitamins: stability during processing and storage10
Colour changes during processing and storage9
Taste: sweet, sour, salty, bitter and umami compounds9
Minerals in foods and their chemistry9
Flavour generation during processing, fermentation and maturation8
Industrial enzymes in food processing8
Maillard reaction and caramelisation8
Phenolic compounds and natural antioxidants8
Enzymatic browning and its control7
Acrylamide and other process contaminants from browning7
Endogenous enzymes in raw materials: lipoxygenase, polyphenol oxidase, peroxidase and pectinases6

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Taste: sweet, sour, salty, bitter and umami compounds · easy

Which receptor pair detects sweet taste?

  1. TRPV1 and TRPM8
  2. T2R receptors
  3. T1R1 and T1R3
  4. T1R2 and T1R3
Answer and working

D. T1R2 and T1R3

T1R2/T1R3 is the sweet receptor; T1R1/T1R3 is umami; T2Rs detect bitter; TRPV1 and TRPM8 detect heat and cooling.

Further reading: Fennema's Food Chemistry

2 · Flavour generation during processing, fermentation and maturation · medium

Black tea 'fermentation' is mainly:

  1. Acetic fermentation of the leaf juice
  2. Enzymatic oxidation of catechins by polyphenol oxidase
  3. Alcoholic fermentation by yeasts on the rolled leaves
  4. Lactic fermentation of the leaf sugars by bacteria
Answer and working

B. Enzymatic oxidation of catechins by polyphenol oxidase

PPO oxidises catechins to theaflavins and thearubigins, giving colour, briskness and flavour.

Further reading: Reineccius, Flavor Chemistry and Technology

3 · Food enzymes: specificity, kinetics, inhibition and coenzymes · medium

An enzyme has Vmax = 100 units and Km = 2 mM. What is the rate at 6 mM substrate?

  1. 300 units
  2. 33 units
  3. 75 units
  4. 50 units
Answer and working

C. 75 units

v = 100 × 6/(2 + 6) = 75. 50 units is the rate at [S] = Km.

Further reading: Handbook of Food Enzymology

4 · Food enzymes: specificity, kinetics, inhibition and coenzymes · medium

In a Lineweaver-Burk plot, what is the y-intercept?

  1. Vmax/Km
  2. −1/Km
  3. 1/Vmax
  4. Km/Vmax
Answer and working

C. 1/Vmax

1/v = (Km/Vmax)(1/[S]) + 1/Vmax: y-intercept 1/Vmax, x-intercept −1/Km, slope Km/Vmax.

Further reading: Handbook of Food Enzymology

5 · Endogenous enzymes in raw materials: lipoxygenase, polyphenol oxidase, peroxidase and pectinases · medium

Why is a little soy flour added to wheat flour in some breads?

  1. Its lipoxygenase bleaches carotenoids, whitening the crumb
  2. Its pectinases soften the crust
  3. Its polyphenol oxidase darkens the crumb
  4. Its trypsin inhibitors slow staling
Answer and working

A. Its lipoxygenase bleaches carotenoids, whitening the crumb

Coupled oxidation by lipoxygenase bleaches flour carotenoids and also aids dough mixing tolerance.

Further reading: Handbook of Food Enzymology

6 · Industrial enzymes in food processing · medium

What is the main advantage of immobilised enzymes?

  1. Higher activity than any free enzyme in every case
  2. No effect of temperature on activity
  3. No need for any pH control in the reactor
  4. The enzyme is reused and the process runs continuously
Answer and working

D. The enzyme is reused and the process runs continuously

Enzymes bound to supports in columns can be used repeatedly; activity may be lower than free enzyme.

Further reading: Handbook of Food Enzymology

7 · Maillard reaction and caramelisation · medium

How does pH affect the rate of the Maillard reaction?

  1. It is not affected by pH at all
  2. It is fastest at pH 1 and stops above pH 5
  3. It is faster at higher pH, where amino groups are unprotonated
  4. It occurs only at exactly neutral pH
Answer and working

C. It is faster at higher pH, where amino groups are unprotonated

The unprotonated amino group is the reactive form, so alkaline conditions speed browning, as in pretzels dipped in lye.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

8 · Maillard reaction and caramelisation · medium

Caramelisation differs from the Maillard reaction because it:

  1. Requires polyphenol oxidase and oxygen to proceed
  2. Produces mainly sulphur compounds
  3. Occurs only at refrigeration temperatures
  4. Involves sugars heated without amino compounds
Answer and working

D. Involves sugars heated without amino compounds

Caramelisation is thermal degradation of sugars, catalysed by acid or base, at higher temperatures.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

9 · Acrylamide and other process contaminants from browning · medium

How does IARC classify acrylamide?

  1. Group 1, carcinogenic to humans with sufficient evidence
  2. Group 2A, probably carcinogenic
  3. Not evaluated by IARC
  4. Group 3, not classifiable at all
Answer and working

B. Group 2A, probably carcinogenic

IARC placed acrylamide in Group 2A in 1994.

Further reading: IARC Monographs Volume 60

10 · Phenolic compounds and natural antioxidants · easy

Which flavonoid subclass includes hesperidin and naringin of citrus?

  1. Flavan-3-ols
  2. Isoflavones
  3. Flavanones
  4. Anthocyanidins
Answer and working

C. Flavanones

Isoflavones are soy compounds; flavan-3-ols are catechins.

Further reading: Fennema's Food Chemistry

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Food Chemistry II: Pigments, Flavours, Vitamins, Minerals, Enzymes & BrowningThe full study manual, free GATEFormat, sections and how it has changed

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