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Australasian Veterinary Examination, formerly the National Veterinary Examination Aquatic Animal Diseases, Health Care & Management: practice questions

Australasian Veterinary Examination (AVE), formerly the National Veterinary Examination (NVE) · Australia / New Zealand · Australasian Veterinary Boards Council (AVBC)

Original practice questions written to the published syllabus · not past papers
10worked here, free
51written to this exam in this subject
10syllabus units

What this subject covers

Diseases of fish and crustaceans, the role of water quality, aquaculture biosecurity, and the residue rules for therapeutants used in food fish.

In the exams: NAVLE aquatics 1%; China runs a separate aquatic-animal category of its national exam

Where the 51 questions fall

How our questions for Australasian Veterinary Examination, formerly the National Veterinary Examination spread across the units of Aquatic Animal Diseases, Health Care & Management. Difficulty: 20 easy, 28 medium, 3 hard.

Water-quality diseases7
Biosecurity in aquaculture7
Parasitic diseases of fish and shrimp6
Bacterial diseases of fish and shrimp5
WOAH-listed aquatic animal diseases5
Therapeutants and residues5
Diagnosis of aquatic animal disease4
Viral diseases of fish and shrimp4
Fungal diseases of fish4
Degree-day withdrawal periods4

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Water-quality diseases · medium

Fresh water at 25 °C holds 8.26 mg/L of dissolved oxygen at saturation. A pond at 25 °C reads 5.0 mg/L. What is the percent saturation?

  1. About 61%
  2. About 165%
  3. About 50%
  4. About 3.3%
Answer and working

A. About 61%

% saturation = 5.0 / 8.26 × 100 = 60.5%, about 61%. 165% inverts the ratio (8.26/5.0); 50% assumes saturation is 10 mg/L; 3.3 is the deficit in mg/L (8.26 − 5.0), not a percentage.

Further reading: Boyd & Tucker, Pond Aquaculture Water Quality Management; Emerson et al. (1975)

2 · Water-quality diseases · hard

Fish in a recirculating system have chocolate-brown blood and gasp despite adequate dissolved oxygen. Which measure directly reduces uptake of the likely toxicant across the gill?

  1. Increasing feeding to improve condition
  2. Adding sodium chloride to raise chloride
  3. Raising the pH
  4. Adding sodium thiosulphate to neutralise the nitrite
Answer and working

B. Adding sodium chloride to raise chloride

Brown blood is methaemoglobinaemia from nitrite. Nitrite enters by the gill chloride-uptake pathway, so raising chloride competitively reduces its uptake. Raising pH increases ammonia toxicity rather than helping; thiosulphate neutralises chlorine; more feed raises ammonia and nitrite production.

Further reading: Roberts (ed.), Fish Pathology, 4th ed.; Noga, Fish Disease: Diagnosis and Treatment, 2nd ed.

3 · Diagnosis of aquatic animal disease · easy

Why are salmonids and carp called physostomous?

  1. They breathe air through a labyrinth organ above the gill chamber
  2. The swim bladder is filled only by a gas gland
  3. The swim bladder stays connected to the gut by a pneumatic duct
  4. They lack a swim bladder
Answer and working

C. The swim bladder stays connected to the gut by a pneumatic duct

Physostomous fish keep a duct from swim bladder to gut and can gulp or release air. A closed bladder filled by a gas gland and rete mirabile is the physoclistous arrangement. Labyrinth organs belong to anabantids such as gouramis.

Further reading: Roberts (ed.), Fish Pathology, 4th ed.; Noga, Fish Disease: Diagnosis and Treatment, 2nd ed.

4 · Diagnosis of aquatic animal disease · easy

For a laboratory investigation of a fish mortality, which animals are the best samples?

  1. Live, moribund fish showing typical signs
  2. Fish found dead the previous evening
  3. Fish that died two days ago and were kept frozen
  4. Only apparently healthy fish
Answer and working

A. Live, moribund fish showing typical signs

Fish tissues autolyse within hours of death and secondary bacteria overgrow the primary pathogen; freezing ruins histology. Moribund fish give the best chance of finding the cause; a few healthy fish are useful only as comparison.

Further reading: Roberts (ed.), Fish Pathology, 4th ed.; Noga, Fish Disease: Diagnosis and Treatment, 2nd ed.

5 · Diagnosis of aquatic animal disease · easy

Which quick farm-side test finds most external parasites of fish?

  1. Faecal flotation in saturated salt solution to find parasite eggs
  2. Serum antibody ELISA on pooled blood samples
  3. Blood culture on blood agar at 37 °C for 48 hours
  4. Wet mount of skin scrapings and a gill clip
Answer and working

D. Wet mount of skin scrapings and a gill clip

A wet mount in the fish's own water, examined within minutes, shows ciliates, flagellates, monogeneans and crustacean parasites. Blood culture at 37 °C is wrong for most fish bacteria; serology and faecal flotation do not detect ectoparasites.

Further reading: Roberts (ed.), Fish Pathology, 4th ed.; Noga, Fish Disease: Diagnosis and Treatment, 2nd ed.

6 · Bacterial diseases of fish and shrimp · medium

Farmed tilapia at 30 °C swim erratically in spirals, with exophthalmia and corneal opacity; the brain yields Gram-positive cocci in chains. What is the likely agent?

  1. Aeromonas salmonicida
  2. Flavobacterium psychrophilum
  3. Streptococcus iniae or S. agalactiae
  4. Vibrio anguillarum
Answer and working

C. Streptococcus iniae or S. agalactiae

Meningoencephalitis with exophthalmia in warm-water tilapia and Gram-positive cocci points to streptococcosis. The other organisms are Gram-negative rods, and F. psychrophilum is a cold-water pathogen.

Further reading: Roberts (ed.), Fish Pathology, 4th ed.; Noga, Fish Disease: Diagnosis and Treatment, 2nd ed.

7 · Fungal diseases of fish · easy

Crayfish plague is caused by:

  1. Saprolegnia parasitica
  2. White spot syndrome virus
  3. Aphanomyces invadans
  4. Aphanomyces astaci
Answer and working

D. Aphanomyces astaci

A. astaci causes crayfish plague, a WOAH-listed disease that devastated native European crayfish. A. invadans causes EUS in fish.

Further reading: WOAH Aquatic Animal Health Code, chapter 1.3; WOAH Manual of Diagnostic Tests for Aquatic Animals

8 · Parasitic diseases of fish and shrimp · easy

A flattened, disc-shaped crustacean moving freely over the skin of pond carp and feeding with a preoral stylet is:

  1. Lernaea (anchor worm)
  2. Argulus
  3. Ergasilus
  4. Gyrodactylus
Answer and working

B. Argulus

Argulus (the fish louse) is a mobile branchiuran. Lernaea females embed their heads in the host; Ergasilus attaches to gills; Gyrodactylus is a monogenean flatworm, not a crustacean.

Further reading: Roberts (ed.), Fish Pathology, 4th ed.; Noga, Fish Disease: Diagnosis and Treatment, 2nd ed.

9 · WOAH-listed aquatic animal diseases · easy

Which one of the following diseases of carp and trout appears on the WOAH list?

  1. Carp pox
  2. Columnaris
  3. Koi herpesvirus disease
  4. Enteric redmouth disease
Answer and working

C. Koi herpesvirus disease

Koi herpesvirus disease is listed. Columnaris, carp pox (cyprinid herpesvirus 1) and enteric redmouth are not.

Further reading: WOAH Aquatic Animal Health Code, chapter 1.3; WOAH Manual of Diagnostic Tests for Aquatic Animals

10 · Therapeutants and residues · medium

Tricaine methanesulfonate is used as a fish anaesthetic. Why is it usually buffered with sodium bicarbonate?

  1. It acidifies the water, especially soft water, which stresses the fish
  2. Bicarbonate prevents its absorption
  3. It raises pH dangerously
  4. It is inactive without bicarbonate, which converts it to its anaesthetic form
Answer and working

A. It acidifies the water, especially soft water, which stresses the fish

Tricaine methanesulfonate is acidic and lowers pH in poorly buffered water, so it is buffered. It is active without buffer; availability and withdrawal requirements vary by country — confirm against the product label and your national formulary.

Further reading: Roberts (ed.), Fish Pathology, 4th ed.; Noga, Fish Disease: Diagnosis and Treatment, 2nd ed.

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Read the subject

Aquatic Animal Diseases, Health Care & ManagementThe full study manual, free Australasian Veterinary Examination, formerly the National Veterinary ExaminationFormat, sections and how it has changed

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Aquatic Animal Diseases, Health Care & Management for other exams

National Practising Veterinarian Qualification Examination · ChinaASRB NET and ARS / SMS / STO · IndiaRCVS Statutory Membership Examination · UKICAR AIEEA · IndiaNAVLE · USA / CanadaENADE · BrazilNational Veterinary Examination · Japan

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