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ICAR AIEEA-PG / PGS Food Chemistry I: Water, Carbohydrates, Proteins & Lipids: practice questions

ICAR AIEEA-PG / PGS — Code 18 Food Science Technology · India · ICAR through the National Testing Agency

Original practice questions written to the published syllabus · not past papers
10worked here, free
81written to this exam in this subject
14syllabus units

What this subject covers

The four constituents that make up most of any food and how they behave during processing and storage: water and its mobility, sugars and starch, proteins and their functional properties, and lipids and their oxidation.

In the exams: GATE XE-G and XL-U Section 1 (carbohydrates, proteins, lipids); ICAR AIEEA-PG Code 18 Unit I; ICAR AICE 35.b Unit 5 (emulsions, foams, gels, functional properties)

What ICAR AIEEA-PG / PGS asks from it

ICAR AIEEA-PG / PGS — Code 18 Food Science Technology

Unit I names general chemistry of food constituents, physical properties, colloidal systems, gels and emulsions, physicochemical changes during processing and storage, and metabolism of the macronutrients. MCQs test definitions and classification: Osborne fractions, reducing and non-reducing sugars, HM and LM pectin, aw limits for microbes, antioxidant classes and the meaning of PV, IV, SV and AV.

Where the 81 questions fall

How our questions for ICAR AIEEA-PG / PGS spread across the units of Food Chemistry I: Water, Carbohydrates, Proteins & Lipids. Difficulty: 28 easy, 48 medium, 5 hard.

Food proteins: classification and sources9
Monosaccharides, disaccharides and oligosaccharides8
Water in foods: structure, bound and free water, sorption isotherms and water activity7
Cellulose, pectic substances, gums and dietary fibre7
Post-mortem changes and tenderisation of muscle proteins7
Lipids: classification, structure and nomenclature7
Amino acids, peptides and protein structure6
Lipid oxidation: autoxidation, photo-oxidation, rancidity and antioxidants6
Hydrolytic rancidity and thermal polymerisation of frying oils6
Starch: structure, gelatinisation, pasting and retrogradation5
Protein denaturation and functional properties: solubility, gelation, emulsification and foaming4
Physical properties of fats: crystallisation and polymorphism4
Chemical and physical changes in macronutrients during processing4
Glass transition and molecular mobility1

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Water in foods: structure, bound and free water, sorption isotherms and water activity · medium

Which group of microorganisms can grow at the lowest water activity?

  1. most spoilage bacteria, which grow down to about aw 0.70 in dry foods
  2. xerophilic moulds and osmophilic yeasts, down to about aw 0.61
  3. Gram-negative pathogens such as Salmonella, which grow down to about aw 0.75
  4. lactic acid bacteria, which grow down to about aw 0.65 in jams and honey
Answer and working

B. xerophilic moulds and osmophilic yeasts, down to about aw 0.61

Xerophilic moulds and osmophilic yeasts are the most tolerant, growing to about 0.61. Most spoilage bacteria need above about 0.90; Salmonella needs about 0.93 or more; lactic acid bacteria are not the low-aw limit.

Further reading: Damodaran and Parkin, Fennema's Food Chemistry

2 · Starch: structure, gelatinisation, pasting and retrogradation · easy

Waxy maize starch contains

  1. about 70 % amylose and 30 % amylopectin
  2. about 25 % amylose like normal maize starch
  3. almost no amylose, being nearly all amylopectin
  4. only amylose with no amylopectin
Answer and working

C. almost no amylose, being nearly all amylopectin

Waxy starches are mutants with almost no amylose. Normal maize is about 25 % amylose; high-amylose types reach 50–70 %.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

3 · Starch: structure, gelatinisation, pasting and retrogradation · easy

Loss of birefringence (the Maltese cross) when starch granules are heated in water indicates

  1. retrogradation
  2. dextrinisation
  3. gelatinisation
  4. syneresis
Answer and working

C. gelatinisation

Gelatinisation melts crystalline order, so birefringence disappears. Retrogradation is re-association on cooling; dextrinisation is dry-heat breakdown; syneresis is weeping of liquid from a gel.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

4 · Cellulose, pectic substances, gums and dietary fibre · medium

Pectin with a degree of esterification of 35 % is classified as

  1. high-methoxyl pectin
  2. amidated high-methoxyl pectin
  3. protopectin
  4. low-methoxyl pectin
Answer and working

D. low-methoxyl pectin

The dividing line is 50 % esterification; 35 % is low-methoxyl. Protopectin is the insoluble parent form in unripe fruit.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

5 · Amino acids, peptides and protein structure · easy

Which of these amino acids is essential for adult humans?

  1. Lysine
  2. Alanine
  3. Glutamic acid
  4. Serine
Answer and working

A. Lysine

Lysine is one of the nine essential amino acids (His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val). Alanine, glutamic acid and serine can be synthesised.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

6 · Amino acids, peptides and protein structure · medium

The covalent cross-link between two cysteine residues that is important in gluten and in egg-white gels is a

  1. peptide bond
  2. hydrogen bond
  3. disulphide bond
  4. glycosidic bond
Answer and working

C. disulphide bond

Oxidation of two cysteine thiol groups forms an S–S disulphide bond. Peptide bonds join the backbone; hydrogen bonds are non-covalent; glycosidic bonds join sugars.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

7 · Food proteins: classification and sources · easy

In the Osborne classification, proteins soluble in about 70 % aqueous ethanol are called

  1. prolamins
  2. albumins
  3. globulins
  4. glutelins
Answer and working

A. prolamins

Prolamins (gliadin, zein, hordein) dissolve in aqueous alcohol. Albumins dissolve in water, globulins in dilute salt, glutelins in dilute acid or alkali.

Further reading: Belitz, Grosch and Schieberle, Food Chemistry

8 · Lipid oxidation: autoxidation, photo-oxidation, rancidity and antioxidants · easy

In autoxidation of lipids, the propagation step is

  1. RH → R• + H• (with heat or light)
  2. R• + R• → R–R
  3. ROOH → RO• + •OH
  4. ROO• + RH → ROOH + R•
Answer and working

D. ROO• + RH → ROOH + R•

Propagation transfers the radical: a peroxyl radical takes hydrogen from another lipid, forming a hydroperoxide and a new alkyl radical. The first equation is initiation, the third termination, the fourth hydroperoxide decomposition.

Further reading: Frankel, Lipid Oxidation

9 · Hydrolytic rancidity and thermal polymerisation of frying oils · easy

The free fatty acid content of an oil, expressed as % oleic acid, is approximately equal to

  1. acid value divided by 1.99
  2. acid value multiplied by 1.99
  3. saponification value divided by 3
  4. iodine value divided by 1.99
Answer and working

A. acid value divided by 1.99

FFA (% oleic) ≈ AV/1.99, from 56.1 (KOH) and 282 (oleic acid): 282/(56.1 × 10) ≈ 0.503 = 1/1.99. Saponification and iodine values measure other things.

Further reading: deMan and others, Principles of Food Chemistry

10 · Chemical and physical changes in macronutrients during processing · medium

Acrylamide forms in fried and baked foods mainly from

  1. lysine and sucrose at refrigeration temperatures
  2. asparagine and reducing sugars at high temperature and low moisture
  3. cysteine and fat during boiling in water
  4. glutamine and starch during freezing
Answer and working

B. asparagine and reducing sugars at high temperature and low moisture

Acrylamide is a Maillard-related product of free asparagine with reducing sugars above about 120 °C in dry conditions. Boiling, freezing and refrigeration do not form it.

Further reading: Damodaran and Parkin, Fennema's Food Chemistry

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Food Chemistry I: Water, Carbohydrates, Proteins & LipidsThe full study manual, free ICAR AIEEA-PG / PGSFormat, sections and how it has changed

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