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ICAR AIEEA-PG Microbiology of Raw & Market Milk: practice questions

ICAR AIEEA-PG: Dairy Science (group 16) and Dairy Technology (group 17) · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
90written to this exam in this subject
12syllabus units

What this subject covers

Where milk bacteria come from, how they grow and spoil milk, and how processing changes the count.

In the exams: ICAR AICE subject 36 Unit 3; ICAR AIEEA-PG group 16 (classification by growth temperature, spoilage, grading of raw milk, bacteriological aspects of processing)

What ICAR AIEEA-PG asks from it

ICAR AIEEA-PG — Dairy Science (group 16)

Classification of milk microbes by growth temperature, spoilage, antimicrobial systems, grading of raw milk (SPC, MBRT, resazurin), mastitic milk, and the bacteriology of thermisation, pasteurisation, boiling, sterilisation, UHT, bactofugation and membrane filtration.

Where the 90 questions fall

How our questions for ICAR AIEEA-PG spread across the units of Microbiology of Raw & Market Milk. Difficulty: 30 easy, 49 medium, 11 hard.

Psychrotrophs, mesophiles, thermodurics and thermophiles10
Microbiological grading of raw milk: SPC, MBRT and resazurin tests9
Bacteriology of thermisation, pasteurisation, boiling, sterilisation and UHT9
Microbial spoilage: souring, curdling, gassiness, ropiness, proteolysis, lipolysis8
Sources of contamination: udder, animal, milker, equipment, water, air7
Natural antimicrobial systems in raw milk; the lactoperoxidase system7
Microflora of mastitic milk7
Effect of chilling and storage on raw milk flora7
Bactofugation and microfiltration for bacteria removal7
Post-pasteurisation contamination7
Psychrotroph enzymes and their survival of heat treatment6
Biofilms in milking and processing equipment6

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Psychrotrophs, mesophiles, thermodurics and thermophiles · easy

Psychrotrophs are defined as bacteria that:

  1. Grow at 7 degrees Celsius or below, whatever their optimum
  2. Have an optimum growth temperature below 15 degrees Celsius
  3. Grow best at 55 degrees Celsius
  4. Survive pasteurisation at 63 degrees Celsius for 30 minutes
Answer and working

A. Grow at 7 degrees Celsius or below, whatever their optimum

The definition is ability to grow at 7 C or below; psychrophiles have optima at 15 C or below; thermodurics survive pasteurisation.

Further reading: Robinson (ed.), Dairy Microbiology Handbook

2 · Psychrotrophs, mesophiles, thermodurics and thermophiles · easy

The laboratory pasteurisation count is made by heating the sample at about:

  1. 121 degrees Celsius for 15 minutes
  2. 4 degrees Celsius for 10 days
  3. 80 degrees Celsius for 10 minutes
  4. 62.8 degrees Celsius for 30 minutes
Answer and working

D. 62.8 degrees Celsius for 30 minutes

62.8 C for 30 min simulates batch pasteurisation; 80 C for 10 min is for spore counts.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

3 · Psychrotroph enzymes and their survival of heat treatment · easy

Psychrotroph enzymes are a problem in UHT milk mainly because they:

  1. Are produced by bacteria growing in UHT milk
  2. Are formed by the UHT heating itself
  3. Partly survive UHT treatment
  4. Are added with packaging material
Answer and working

C. Partly survive UHT treatment

Enzymes produced in raw milk survive heat and act in storage; the bacteria are killed.

Further reading: Robinson (ed.), Dairy Microbiology Handbook

4 · Microflora of mastitic milk · easy

Which mastitis pathogen is classed as contagious?

  1. Staphylococcus aureus
  2. Klebsiella pneumoniae
  3. Escherichia coli
  4. Streptococcus uberis
Answer and working

A. Staphylococcus aureus

S. aureus spreads cow to cow at milking; the others are environmental.

Further reading: Robinson (ed.), Dairy Microbiology Handbook

5 · Microbiological grading of raw milk: SPC, MBRT and resazurin tests · easy

Under the international standard, the plate count of milk is incubated at:

  1. 7 degrees Celsius for 10 days
  2. 37 degrees Celsius for 24 hours
  3. 30 degrees Celsius for 72 hours
  4. 55 degrees Celsius for 48 hours
Answer and working

C. 30 degrees Celsius for 72 hours

ISO uses 30 C for 72 h; US methods use 32 C for 48 h.

Further reading: Robinson (ed.), Dairy Microbiology Handbook

6 · Microbiological grading of raw milk: SPC, MBRT and resazurin tests · easy

In the methylene blue reduction test, a longer reduction time indicates:

  1. Better quality milk
  2. More bacteria in the milk
  3. Higher fat content
  4. Added water
Answer and working

A. Better quality milk

Fewer active bacteria lower the redox potential more slowly.

Further reading: Robinson (ed.), Dairy Microbiology Handbook

7 · Effect of chilling and storage on raw milk flora · medium

A raw milk count rises from 1,000 to 16,000 cfu per millilitre in 24 hours. What is the generation time?

  1. 16 hours
  2. 4 hours
  3. 1.5 hours
  4. 6 hours
Answer and working

D. 6 hours

16-fold = 4 generations in 24 h, so g = 6 h. 4 is the number of generations.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

8 · Bacteriology of thermisation, pasteurisation, boiling, sterilisation and UHT · easy

The 72 degrees Celsius for 15 seconds pasteurisation standard was confirmed to inactivate which more heat-resistant pathogen?

  1. Coxiella burnetii
  2. Geobacillus stearothermophilus
  3. Bacillus cereus spores
  4. Clostridium botulinum spores
Answer and working

A. Coxiella burnetii

Coxiella, the Q-fever agent, is slightly more heat-resistant than M. tuberculosis; spores survive pasteurisation.

Further reading: Robinson (ed.), Dairy Microbiology Handbook

9 · Bactofugation and microfiltration for bacteria removal · medium

Bactofugation of cheese milk mainly prevents:

  1. Early souring by Lactococcus
  2. Late blowing by Clostridium tyrobutyricum
  3. Ropiness by Alcaligenes
  4. Bitty cream caused by Bacillus cereus spores
Answer and working

B. Late blowing by Clostridium tyrobutyricum

Removing clostridial spores reduces or replaces the need for nitrate or lysozyme.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

10 · Post-pasteurisation contamination · medium

Which is the main factor limiting the refrigerated shelf life of conventional pasteurised milk?

  1. Fat oxidation
  2. Post-pasteurisation contamination
  3. Lactose crystallisation
  4. Spores in the raw milk that survive heating
Answer and working

B. Post-pasteurisation contamination

Recontamination with psychrotrophs usually decides shelf life.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

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Read the subject

Microbiology of Raw & Market MilkThe full study manual, free ICAR AIEEA-PGFormat, sections and how it has changed

More ICAR AIEEA-PG practice

Starter Cultures & Lactic Acid BacteriaDairy Pathogens, Toxins & Microbial RiskChemistry of Dairy ProductsInfant Formula & Nutritional Dairy ProductsLactose, Milk Salts & VitaminsDairy Analysis & Instrumental MethodsMilk Proteins & EnzymesEngineering Fundamentals for Dairy TechnologistsMicrobiology of Dairy ProductsMilk LipidsProbiotics, Prebiotics & Functional Dairy FoodsDairy Colloids: Emulsions, Foams & GelsGeneral MicrobiologyFluid Flow, Thermodynamics & Heat Transfer in Dairy PlantsMilk Composition & Physical PropertiesSensory Evaluation & Judging of Dairy ProductsBiochemistry for Dairy ScienceComputing, Data & Digital Tools for DairyDairy Animal ScienceDairy Plant Design, Layout & Project EngineeringMarket Milk ProcessingHuman Nutrition & Dairy in the DietDairy Industry & Milk Production SystemsCream, Butter, Ghee & Milk Fat Products

Microbiology of Raw & Market Milk for other exams

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