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ICAR AIEEA-PG / PGS Alternative Proteins & Future Foods: practice questions

ICAR AIEEA-PG / PGS — Code 18 Food Science Technology · India · ICAR through the National Testing Agency

Original practice questions written to the published syllabus · not past papers
10worked here, free
17written to this exam in this subject
5syllabus units

What this subject covers

The newest part of the field: plant-based meat, dairy and egg analogues, cultivated meat, precision-fermentation ingredients, mycoprotein, insects and algae, and 3D food printing — their technology, nutrition, safety, regulation and sustainability claims.

In the exams: ICAR AICE 35.a Unit 2 (dairy analogues based on plant milk); PG research and industry R&D roles

Where the 17 questions fall

How our questions for ICAR AIEEA-PG / PGS spread across the units of Alternative Proteins & Future Foods. Difficulty: 7 easy, 9 medium, 1 hard.

Plant-based meat analogues: ingredients and structuring6
Nutrition and safety of novel proteins5
Plant-based dairy and egg analogues4
Drivers for alternative proteins1
Mycoprotein and fungal biomass1

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Plant-based meat analogues: ingredients and structuring · easy

Textured vegetable protein (TVP) used in mince-type products is made mainly by

  1. High-moisture extrusion
  2. Low-moisture extrusion
  3. Freeze drying
  4. Spray drying
Answer and working

B. Low-moisture extrusion

Low-moisture extrusion expands protein into porous pieces that are dried and rehydrated. High-moisture extrusion gives dense fibrous products; drying methods do not texturise.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

2 · Plant-based meat analogues: ingredients and structuring · medium

Methylcellulose is used in plant-based burgers mainly because it

  1. Gels on heating and binds
  2. Adds red colour that browns
  3. Supplies essential amino acids
  4. Lowers the sodium content
Answer and working

A. Gels on heating and binds

Methylcellulose forms a gel when heated, holding the patty together and giving bite during cooking. Colour comes from beetroot or haem proteins, and methylcellulose provides no protein or sodium reduction.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

3 · Plant-based meat analogues: ingredients and structuring · easy

Which fat is commonly used in plant-based meat analogues to imitate the melting of animal fat?

  1. Fish oil
  2. Coconut oil
  3. Mineral oil
  4. Linseed oil
Answer and working

B. Coconut oil

Coconut oil is solid at room temperature and melts on cooking, imitating animal fat. Fish oil is not plant-based, mineral oil is not a food fat, and linseed oil is liquid and oxidises easily.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

4 · Plant-based meat analogues: ingredients and structuring · hard

A protein isolate differs from a protein concentrate in that the isolate

  1. Is made only by dry fractionation
  2. Has a higher protein content
  3. Contains more starch and fibre
  4. Is always more functional
Answer and working

B. Has a higher protein content

Isolates commonly contain about 80–90% protein, concentrates about 50–70%. Isolates are usually made by wet extraction, contain less starch and fibre, and are not always more functional.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

5 · Plant-based dairy and egg analogues · medium

Unless fortified, plant-based drinks are typically low in

  1. Calcium and vitamin B12
  2. Water and carbohydrate
  3. Dietary fibre only
  4. Energy and sugar
Answer and working

A. Calcium and vitamin B12

Cow milk supplies calcium, vitamin B12, riboflavin and iodine, which plant drinks lack unless added. They are mostly water and may contain sugars and some fibre.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

6 · Mycoprotein and fungal biomass · easy

Tempeh, a traditional fungal protein food, is made with

  1. Rhizopus
  2. Fusarium
  3. Penicillium
  4. Lactobacillus
Answer and working

A. Rhizopus

Tempeh is soybeans bound by Rhizopus mycelium. Fusarium gives mycoprotein, Penicillium ripens cheeses, and Lactobacillus is a bacterium.

Further reading: Wiebe, Applied Microbiology and Biotechnology (2002)

7 · Nutrition and safety of novel proteins · medium

A protein's limiting amino acid score is 0.85 and its digestibility is 0.90. Its PDCAAS is

  1. 0.85
  2. 0.94
  3. 1.75
  4. 0.77
Answer and working

D. 0.77

PDCAAS = 0.85 × 0.90 = 0.765 ≈ 0.77. 0.85 ignores digestibility; 0.94 divides 0.85 by 0.90; 1.75 adds the two values.

Further reading: FAO, Dietary protein quality evaluation in human nutrition (2013)

8 · Nutrition and safety of novel proteins · medium

Digestible lysine is 48 mg/g protein and the reference requirement is 57 mg/g; lysine is limiting. The DIAAS is about

  1. 119
  2. 84
  3. 48
  4. 57
Answer and working

B. 84

DIAAS = 100 × 48 ÷ 57 = 84. 119 inverts the ratio; 48 and 57 are the inputs.

Further reading: FAO, Dietary protein quality evaluation in human nutrition (2013)

9 · Nutrition and safety of novel proteins · medium

DIAAS differs from PDCAAS mainly in that DIAAS

  1. Ileal digestibility per amino acid
  2. Uses faecal crude protein digestibility
  3. Is always truncated at 100
  4. Ignores amino acid composition
Answer and working

A. Ileal digestibility per amino acid

DIAAS uses true ileal digestibility of each indispensable amino acid and is not truncated; PDCAAS uses faecal protein digestibility and truncates at 1.0. Both depend on amino acid composition.

Further reading: FAO, Dietary protein quality evaluation in human nutrition (2013)

10 · Nutrition and safety of novel proteins · easy

Cereal proteins are usually limited by

  1. Lysine
  2. Methionine
  3. Tryptophan only
  4. Leucine
Answer and working

A. Lysine

Cereals are typically lysine-limited, while legumes are limited by sulphur amino acids (methionine and cysteine); combining them improves quality.

Further reading: FAO, Dietary protein quality evaluation in human nutrition (2013)

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Read the subject

Alternative Proteins & Future FoodsThe full study manual, free ICAR AIEEA-PG / PGSFormat, sections and how it has changed

More ICAR AIEEA-PG / PGS practice

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