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ICAR AICE-JRF/SRF Alternative Proteins & Future Foods: practice questions

ICAR AICE-JRF/SRF (PhD) — Food Processing Technology, Food Safety & Quality, Processing and Food Engineering · India · ICAR through the National Testing Agency

Original practice questions written to the published syllabus · not past papers
10worked here, free
98written to this exam in this subject
12syllabus units

What this subject covers

The newest part of the field: plant-based meat, dairy and egg analogues, cultivated meat, precision-fermentation ingredients, mycoprotein, insects and algae, and 3D food printing — their technology, nutrition, safety, regulation and sustainability claims.

In the exams: ICAR AICE 35.a Unit 2 (dairy analogues based on plant milk); PG research and industry R&D roles

Where the 98 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Alternative Proteins & Future Foods. Difficulty: 18 easy, 54 medium, 26 hard.

Plant-based meat analogues: ingredients and structuring9
Nutrition and safety of novel proteins9
Drivers for alternative proteins8
Plant-based dairy and egg analogues8
Mycoprotein and fungal biomass8
Precision fermentation for food proteins and fats8
Cultivated meat: cell lines, media and scaffolds8
Edible insects8
Algae and seaweed as food8
3D food printing8
Regulation of novel and cell-based foods8
Life-cycle assessment and sustainability claims8

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Plant-based meat analogues: ingredients and structuring · easy

Textured vegetable protein (TVP) used in mince-type products is made mainly by

  1. High-moisture extrusion
  2. Low-moisture extrusion
  3. Freeze drying
  4. Spray drying
Answer and working

B. Low-moisture extrusion

Low-moisture extrusion expands protein into porous pieces that are dried and rehydrated. High-moisture extrusion gives dense fibrous products; drying methods do not texturise.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

2 · Plant-based meat analogues: ingredients and structuring · hard

Compared with the beef patties they replace, many plant-based burgers are higher in

  1. Vitamin B12 naturally
  2. Haem iron
  3. Cholesterol
  4. Sodium
Answer and working

D. Sodium

Many analogues contain substantial added salt. Plants contain no vitamin B12 or haem iron naturally (they must be added), and plant-based products contain no cholesterol.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

3 · Plant-based dairy and egg analogues · medium

Unless fortified, plant-based drinks are typically low in

  1. Calcium and vitamin B12
  2. Water and carbohydrate
  3. Dietary fibre only
  4. Energy and sugar
Answer and working

A. Calcium and vitamin B12

Cow milk supplies calcium, vitamin B12, riboflavin and iodine, which plant drinks lack unless added. They are mostly water and may contain sugars and some fibre.

Further reading: Kyriakopoulou, Keppler and van der Goot, Foods (2021)

4 · Precision fermentation for food proteins and fats · easy

Precision fermentation means

  1. Fermenting food at exact temperatures
  2. Growing whole animal tissues
  3. Fermenting vegetables with salt
  4. Engineered microbes, one product
Answer and working

D. Engineered microbes, one product

In precision fermentation a microorganism carries a gene for a target protein or other molecule, which is produced and purified. Temperature control, tissue culture and salt fermentation are other processes.

Further reading: FAO and WHO, Food safety aspects of cell-based food (2023)

5 · Edible insects · hard

Crickets need 2.1 kg feed per kg live weight with 80% edible; cattle need 8.0 kg feed per kg live weight with 40% edible. For 10 kg of edible product, the feed needed is about

  1. 21 kg and 80 kg
  2. 26 kg and 200 kg
  3. 17 kg and 32 kg
  4. 26 kg and 80 kg
Answer and working

B. 26 kg and 200 kg

Crickets: 10 ÷ 0.8 × 2.1 = 26.3 kg; cattle: 10 ÷ 0.4 × 8.0 = 200 kg. 21 and 80 ignore the edible fraction; 17 and 32 multiply by the edible fraction instead of dividing. Real ratios vary widely by system.

Further reading: van Huis et al., Edible insects (FAO, 2013)

6 · Edible insects · hard

Many jurisdictions prohibit rearing food insects on manure mainly because

  1. Insects will not eat manure
  2. Pathogens and residues transfer
  3. Manure lowers insect protein to zero
  4. Manure is too expensive
Answer and working

B. Pathogens and residues transfer

Substrates can transfer pathogens, veterinary drug residues and heavy metals into insects, so substrates for food insects are controlled. Many insects do consume manure, and cost is not the reason.

Further reading: van Huis et al., Edible insects (FAO, 2013)

7 · Nutrition and safety of novel proteins · medium

A protein's limiting amino acid score is 0.85 and its digestibility is 0.90. Its PDCAAS is

  1. 0.85
  2. 0.94
  3. 1.75
  4. 0.77
Answer and working

D. 0.77

PDCAAS = 0.85 × 0.90 = 0.765 ≈ 0.77. 0.85 ignores digestibility; 0.94 divides 0.85 by 0.90; 1.75 adds the two values.

Further reading: FAO, Dietary protein quality evaluation in human nutrition (2013)

8 · Nutrition and safety of novel proteins · easy

Cereal proteins are usually limited by

  1. Lysine
  2. Methionine
  3. Tryptophan only
  4. Leucine
Answer and working

A. Lysine

Cereals are typically lysine-limited, while legumes are limited by sulphur amino acids (methionine and cysteine); combining them improves quality.

Further reading: FAO, Dietary protein quality evaluation in human nutrition (2013)

9 · Nutrition and safety of novel proteins · hard

Plant-based burgers should carry storage and cooking instructions like raw meat mainly because they

  1. Contain live animal cells
  2. Are sterile until opened
  3. Contain no water
  4. Support pathogens
Answer and working

D. Support pathogens

Minced, moist, near-neutral, protein-rich products support pathogens and spoilage much as raw meat does, so cold chain and cooking guidance apply. They are not sterile and contain no animal cells.

Further reading: FAO, Dietary protein quality evaluation in human nutrition (2013)

10 · Life-cycle assessment and sustainability claims · medium

In LCA, a 'cradle-to-gate' study stops at

  1. The consumer's plate
  2. The landfill
  3. The factory gate
  4. The farm gate
Answer and working

C. The factory gate

Cradle-to-gate covers raw materials to the product leaving the factory; cradle-to-grave adds distribution, use and disposal. Farm gate is a narrower agricultural boundary.

Further reading: ISO 14040 and ISO 14044

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Read the subject

Alternative Proteins & Future FoodsThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

More ICAR AICE-JRF/SRF practice

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