LAISHA
Open Laisha

ICAR AIEEA-PG Fermentation Technology & Dairy Biotechnology: practice questions

ICAR AIEEA-PG: Dairy Science (group 16) and Dairy Technology (group 17) · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
30written to this exam in this subject
7syllabus units

What this subject covers

Bioreactors and fermentation processes, recombinant enzymes, precision fermentation and the biotechnology now reshaping dairy ingredients.

In the exams: ICAR AICE subject 36 Units 2 and 7 and subject 37 Unit 7

Where the 30 questions fall

How our questions for ICAR AIEEA-PG spread across the units of Fermentation Technology & Dairy Biotechnology. Difficulty: 11 easy, 18 medium, 1 hard.

Microbial rennet and fermentation-produced chymosin7
Lactose hydrolysis: batch and continuous6
Single-cell protein and value from whey6
Enzyme-modified cheese5
Fermenter design and types of fermentation3
Production of enzymes: beta-galactosidase, proteases, lipases2
Industrial production of lactic acid and other organic acids1

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Industrial production of lactic acid and other organic acids · easy

Fermentation is preferred over chemical synthesis for lactic acid used in poly-lactic acid because fermentation:

  1. Always costs nothing
  2. Gives optically pure L- or D-lactic acid
  3. Produces a racemic mixture suited to polymer crystallinity
  4. Uses hydrogen cyanide
Answer and working

B. Gives optically pure L- or D-lactic acid

Chemical synthesis from acetaldehyde and HCN gives racemic DL-lactic acid.

Further reading: Fox et al., Fundamentals of Cheese Science, 2nd ed.

2 · Production of enzymes: beta-galactosidase, proteases, lipases · easy

Yeast lactase from Kluyveromyces lactis is suited to milk because its pH optimum is:

  1. Strongly acid, 1–2
  2. Strongly alkaline, above 10
  3. Near neutral, 6.5–7
  4. Acidic, 3.5–5
Answer and working

C. Near neutral, 6.5–7

Neutral yeast lactases suit milk; acid fungal lactases suit acid whey.

Further reading: Stanbury, Whitaker and Hall, Principles of Fermentation Technology, 3rd ed.

3 · Production of enzymes: beta-galactosidase, proteases, lipases · medium

Fungal lactases from Aspergillus are preferred for acid whey because they:

  1. Clot milk
  2. Are intracellular
  3. Need potassium ions
  4. Work at acid pH
Answer and working

D. Work at acid pH

Their pH optimum of 3.5–5 matches acid whey; they are extracellular and robust.

Further reading: Fox et al., Fundamentals of Cheese Science, 2nd ed.

4 · Microbial rennet and fermentation-produced chymosin · easy

The most widely used fungal (microbial) coagulant comes from:

  1. Rhizomucor miehei
  2. Aspergillus oryzae
  3. Trichoderma reesei
  4. Penicillium camemberti
Answer and working

A. Rhizomucor miehei

R. miehei protease is the main microbial coagulant; R. pusillus and C. parasitica are others.

Further reading: Stanbury, Whitaker and Hall, Principles of Fermentation Technology, 3rd ed.

5 · Microbial rennet and fermentation-produced chymosin · medium

Compared with chymosin, microbial coagulants tend to:

  1. Work only on goat milk
  2. Be more proteolytic, risking yield loss and bitterness
  3. Contain pepsin
  4. Have a higher clotting-to-proteolytic ratio than calf chymosin
Answer and working

B. Be more proteolytic, risking yield loss and bitterness

Higher general proteolysis loses protein to whey and can make long-ripened cheese bitter.

Further reading: Fox et al., Fundamentals of Cheese Science, 2nd ed.

6 · Microbial rennet and fermentation-produced chymosin · medium

Fermentation-produced chymosin is identical to calf chymosin in:

  1. Animal origin
  2. Pepsin content
  3. Cost per litre
  4. Amino acid sequence
Answer and working

D. Amino acid sequence

It is the calf enzyme made from the cloned prochymosin gene, without pepsin.

Further reading: Fox et al., Fundamentals of Cheese Science, 2nd ed.

7 · Enzyme-modified cheese · easy

Enzyme-modified cheese is typically how much more intense in flavour than natural cheese?

  1. 1000 times
  2. Equal
  3. 15–30 times
  4. 1.2 times
Answer and working

C. 15–30 times

Its concentrated flavour lets small amounts replace aged cheese in foods.

Further reading: Stanbury, Whitaker and Hall, Principles of Fermentation Technology, 3rd ed.

8 · Enzyme-modified cheese · medium

The slurry is pasteurised before enzymes are added in enzyme-modified cheese manufacture to:

  1. Destroy contaminants and native enzymes
  2. Raise the pH
  3. Concentrate solids
  4. Add flavour
Answer and working

A. Destroy contaminants and native enzymes

Control of the incubation depends on starting from a clean, defined substrate.

Further reading: Stanbury, Whitaker and Hall, Principles of Fermentation Technology, 3rd ed.

9 · Single-cell protein and value from whey · medium

Nisin is produced commercially by fermenting milk-based media with:

  1. Aspergillus niger
  2. Propionibacterium freudenreichii
  3. Lactococcus lactis
  4. Bacillus subtilis
Answer and working

C. Lactococcus lactis

Nisin is a lantibiotic made by certain L. lactis strains.

Further reading: Stanbury, Whitaker and Hall, Principles of Fermentation Technology, 3rd ed.

10 · Single-cell protein and value from whey · hard

A cheese plant far from markets with small whey volumes is choosing a whey route. The most defensible first option is often:

  1. Anaerobic digestion or feed use
  2. A lactose refinery
  3. A protein isolate plant
  4. Precision fermentation
Answer and working

A. Anaerobic digestion or feed use

Scale and distance determine viability; high-value routes need large volumes and markets.

Further reading: Stanbury, Whitaker and Hall, Principles of Fermentation Technology, 3rd ed.

Ten here, 30 in the app. The Laisha app has every practice question we have written to ICAR AIEEA-PG in this subject, marked as you go, with timed papers, a jump-to-unanswered list and a certificate when you pass at 70 per cent.

Free for ten days, then one subscription of US$10 a month. We would rather say so here than have you find it later.

Practise the full set in Laisha →

Read the subject

Fermentation Technology & Dairy BiotechnologyThe full study manual, free ICAR AIEEA-PGFormat, sections and how it has changed

More ICAR AIEEA-PG practice

Starter Cultures & Lactic Acid BacteriaDairy Pathogens, Toxins & Microbial RiskMicrobiology of Raw & Market MilkChemistry of Dairy ProductsInfant Formula & Nutritional Dairy ProductsLactose, Milk Salts & VitaminsDairy Analysis & Instrumental MethodsMilk Proteins & EnzymesEngineering Fundamentals for Dairy TechnologistsMicrobiology of Dairy ProductsMilk LipidsProbiotics, Prebiotics & Functional Dairy FoodsDairy Colloids: Emulsions, Foams & GelsGeneral MicrobiologyFluid Flow, Thermodynamics & Heat Transfer in Dairy PlantsMilk Composition & Physical PropertiesSensory Evaluation & Judging of Dairy ProductsBiochemistry for Dairy ScienceComputing, Data & Digital Tools for DairyDairy Animal ScienceDairy Plant Design, Layout & Project EngineeringMarket Milk ProcessingHuman Nutrition & Dairy in the DietDairy Industry & Milk Production Systems

Fermentation Technology & Dairy Biotechnology for other exams

ICAR AICE-JRF/SRF · IndiaASRB NET and ARS · India

Ask Laisha about this

Opens Laisha's chat with your question ready to send. Sign in or start free.