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ICAR AICE-JRF/SRF Cheese Science & Technology: practice questions

ICAR AICE-JRF/SRF (PhD): Dairy Chemistry, Dairy Microbiology, Dairy Technology, Dairy Engineering · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
64written to this exam in this subject
13syllabus units

What this subject covers

Cheese from milk selection to ripening and processed cheese: coagulation, curd handling, salting, ripening, yield and the major variety families.

In the exams: ICAR AICE subjects 34 Unit 7, 36 Unit 5 and 37 Units 1 and 7; ICAR AIEEA-PG group 16 (rennet coagulation and ripening) and group 17 Unit I; ACS Certified Cheese Professional (cheesemaking, ripening); Wisconsin Master Cheesemaker coursework

What ICAR AICE-JRF/SRF asks from it

ICAR AICE-JRF/SRF (PhD) — Dairy Chemistry, Dairy Microbiology, Dairy Technology

Subject 34 Unit 7 (rennet action, changes during manufacture and ripening, coagulants from microbial, animal and plant sources), subject 36 Unit 5 (cheese starters, accelerated ripening, spoilage) and subject 37 Units 1 and 7 (manufacture, microbial rennet and recombinant chymosin, immobilised enzymes, enzyme-modified cheese). Questions go deeper: proteolytic agents and their substrates, Fitz equation, C/P ratio, UF cheese making.

Where the 64 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Cheese Science & Technology. Difficulty: 2 easy, 43 medium, 19 hard.

Ripening and accelerated ripening10
Coagulants: calf rennet, microbial, fermentation-produced chymosin, plant9
Rennet coagulation and gel formation7
Milk quality and pre-treatment for cheese7
Mechanised and UF-based cheese making6
Processed cheese and cheese spreads: emulsifying salts4
Cheddaring, pasta filata stretching and other curd treatments4
Cheese yield: formulas and factors3
Cutting, cooking and syneresis3
Salting: dry, brine and its effect3
Varieties: Cheddar, Gouda, Swiss types, Mozzarella, Parmesan, blue and soft cheeses3
Cheese defects and grading3
Fresh cheeses: cottage, cream cheese, quark, queso fresco, paneer2

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Mechanised and UF-based cheese making · medium

Ultrafiltration of 1,200 kg of milk (3.3% protein) gives 240 kg of retentate. Assuming complete protein retention, what is the protein content of the retentate?

  1. 13.2%
  2. 0.66%
  3. 16.5%
  4. 19.8%
Answer and working

C. 16.5%

Concentration factor = 1200 ÷ 240 = 5; protein = 3.3 × 5 = 16.5%. 13.2% uses the permeate-to-retentate ratio (4); 0.66% divides by the factor; 19.8% counts permeate and retentate together as the multiplier.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

2 · Processed cheese and cheese spreads: emulsifying salts · hard

A processed-cheese batch uses 400 kg of natural cheese (38% moisture) and 10 kg of dry emulsifying salt. How much water must be added for a final moisture of 45%, assuming no evaporation?

  1. 59.1 kg
  2. 163.3 kg
  3. 28.0 kg
  4. 69.1 kg
Answer and working

A. 59.1 kg

Solids = 400 × 0.62 + 10 = 258 kg; final mass = 258 ÷ 0.55 = 469.1 kg; water = 469.1 − 400 − 10 = 59.1 kg. 69.1 kg forgets the salt already in the batch; 163.3 kg treats 45% as the solids content; 28.0 kg just multiplies the moisture difference by the cheese mass.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

3 · Coagulants: calf rennet, microbial, fermentation-produced chymosin, plant · hard

A cheesemaker switches from calf chymosin to a more proteolytic microbial coagulant at the same clotting dose for long-ripened Cheddar. What is the most likely result?

  1. No effect, since the clotting dose was matched exactly
  2. Faster lactose fermentation by the coagulant and a sharp rise in acidity in the vat
  3. A large rise in yield because more casein stays in the curd
  4. A small fall in yield and more risk of bitterness during ripening
Answer and working

D. A small fall in yield and more risk of bitterness during ripening

More non-specific proteolysis releases more casein fragments into the whey and leaves more hydrophobic peptides in the cheese. Matching the clotting dose does not match the proteolytic activity; coagulants do not ferment lactose.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

4 · Rennet coagulation and gel formation · medium

After chymosin action, which kappa-casein fragment leaves with the whey?

  1. Caseinomacropeptide (residues 106–169)
  2. Gamma-1-casein fragment (residues 29–209)
  3. Para-kappa-casein (residues 1–105)
  4. Alpha-s1-I casein (residues 24–199)
Answer and working

A. Caseinomacropeptide (residues 106–169)

The hydrophilic C-terminal caseinomacropeptide is released into the serum; para-kappa-casein stays on the micelle. Gamma-caseins come from plasmin action on beta-casein; alpha-s1-I comes from chymosin action during ripening.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

5 · Cutting, cooking and syneresis · medium

Why is part of the whey replaced by water during Gouda making?

  1. To add calcium from the water so the curd fuses more firmly in the press
  2. To lower the fat content of the curd so that it meets the fat-in-dry-matter limit set by law
  3. To dilute the coagulant so that it stays active for longer in the curd
  4. To remove lactose so that less acid forms and the cheese keeps a higher pH
Answer and working

D. To remove lactose so that less acid forms and the cheese keeps a higher pH

Washing curd removes lactose, limiting lactic acid and giving the higher pH and elastic body of Gouda, Edam and Colby. It does not lower fat or add useful calcium.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

6 · Cheddaring, pasta filata stretching and other curd treatments · medium

Why will Mozzarella curd not stretch if its pH is too high?

  1. Too much colloidal calcium phosphate has dissolved, so the curd turns to liquid
  2. Too much salt has been absorbed from the cooking water, firming the curd
  3. Too little calcium phosphate has dissolved, so the curd is not plastic
  4. The coagulant has been destroyed, so the protein network cannot form fibres
Answer and working

C. Too little calcium phosphate has dissolved, so the curd is not plastic

Stretching needs partial demineralisation, reached in a pH window of about 5.2–5.4 for cow-milk Mozzarella. Too low a pH gives sticky, soupy curd with fat loss.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

7 · Salting: dry, brine and its effect · hard

A Cheddar has low salt-in-moisture. Which ripening defect is most likely?

  1. Mould growth inside the cheese, because a low salt level draws oxygen into the paste
  2. Late blowing by clostridia, because salt has no role in spore control
  3. Bitter, acid, pasty cheese from excessive starter and coagulant activity
  4. Dry, crumbly, slow-ripening cheese with a pronounced salty taste
Answer and working

C. Bitter, acid, pasty cheese from excessive starter and coagulant activity

Low S/M permits too much starter acid and coagulant proteolysis, giving bitterness and pasty body. High S/M gives slow, dry, salty cheese.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

8 · Ripening and accelerated ripening · hard

Why are exogenous proteinases added to milk a poor way to accelerate Cheddar ripening?

  1. Most is lost in whey; what stays risks bitterness and lower yield
  2. Proteinases are not legal in any country as food processing aids
  3. Proteinases ferment lactose and make the curd far too acid to press
  4. Proteinases are destroyed by salt, so they never reach the cheese interior
Answer and working

A. Most is lost in whey; what stays risks bitterness and lower yield

Enzyme added to milk mostly leaves with the whey, early proteolysis lowers yield and raises bitterness risk, and curd addition is hard to distribute evenly; encapsulation was developed to address this. Proteinases do not ferment lactose.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

9 · Varieties: Cheddar, Gouda, Swiss types, Mozzarella, Parmesan, blue and soft cheeses · medium

Small eyes in Gouda and Edam are produced mainly by:

  1. Air trapped mechanically during dry salting of curd
  2. Yeasts on the rind producing gas that diffuses inward
  3. Citrate-fermenting mesophilic starter strains producing CO2
  4. Propionibacteria held in a warm room at about 22 °C for weeks
Answer and working

C. Citrate-fermenting mesophilic starter strains producing CO2

Dutch-type starters include citrate-fermenting Lactococcus and Leuconostoc strains whose CO2 forms a few small eyes. Propionic eyes are typical of Swiss types.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

10 · Processed cheese and cheese spreads: emulsifying salts · medium

Which group lists commonly used emulsifying salts for processed cheese?

  1. Sodium citrates and sodium mono-, di- and polyphosphates
  2. Sodium benzoate, potassium sorbate and nisin only
  3. Calcium chloride, calcium lactate and calcium carbonate salts
  4. Sodium nitrate, potassium nitrate and lysozyme
Answer and working

A. Sodium citrates and sodium mono-, di- and polyphosphates

Citrates and phosphates are the main emulsifying salts. Calcium salts would do the opposite; nitrates and lysozyme are anti-clostridial; sorbate and benzoate are preservatives.

Further reading: Fox, Guinee, Cogan and McSweeney, Fundamentals of Cheese Science

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Read the subject

Cheese Science & TechnologyThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

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Cheese Science & Technology for other exams

ICAR AIEEA-PG · IndiaCertified Cheese Professional · USA

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