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ICAR AICE-JRF/SRF Emerging & Non-thermal Dairy Processing: practice questions

ICAR AICE-JRF/SRF (PhD): Dairy Chemistry, Dairy Microbiology, Dairy Technology, Dairy Engineering · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
74written to this exam in this subject
12syllabus units

What this subject covers

Alternatives and additions to heat: high pressure, pulsed electric fields, ultrasound, ohmic and microwave heating, irradiation, carbonation and hurdle technology.

In the exams: ICAR AICE subject 37 Unit 3 and subject 35a Unit 1; ICAR AICE subject 36 Unit 4

Where the 74 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Emerging & Non-thermal Dairy Processing. Difficulty: 19 easy, 46 medium, 9 hard.

High pressure processing10
Ohmic, microwave, radio-frequency and infrared heating9
Pulsed electric fields8
Regulatory approval of novel processes8
Irradiation and UV-C treatment of milk7
Cold plasma7
Supercritical fluid extraction7
Ultrasound in processing and cleaning6
Hurdle technology5
Microencapsulation and nanotechnology3
Carbonation and carbon dioxide to extend shelf life2
3D printing and other new formats2

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · High pressure processing · easy

What pressure range is most used commercially in high pressure processing?

  1. 4–6 MPa
  2. 400–600 MPa
  3. 40–60 kPa
  4. 4,000–6,000 MPa
Answer and working

B. 400–600 MPa

Commercial HPP mostly runs at 400–600 MPa for a few minutes.

Further reading: Barbosa-Cánovas et al., Nonthermal Preservation of Foods

2 · High pressure processing · easy

Which microorganisms are most resistant to high pressure alone at ambient temperature?

  1. Bacterial spores
  2. Moulds
  3. Yeasts
  4. Gram-negative rods
Answer and working

A. Bacterial spores

Spores need pressure combined with heat; Gram-negatives are generally more pressure sensitive than Gram-positives.

Further reading: Barbosa-Cánovas et al., Nonthermal Preservation of Foods

3 · Pulsed electric fields · medium

What is the mechanism of microbial inactivation by pulsed electric fields?

  1. Dissolving cell walls with ozone
  2. Heating the cell interior to over 100 °C
  3. Electroporation of the cell membrane
  4. Breaking covalent bonds in DNA directly
Answer and working

C. Electroporation of the cell membrane

An induced transmembrane potential above about 1 V creates pores, irreversible at high intensity.

Further reading: Barbosa-Cánovas et al., Nonthermal Preservation of Foods

4 · Ultrasound in processing and cleaning · easy

What frequency range is typical of power ultrasound for processing?

  1. About 2–10 GHz
  2. About 2–10 MHz
  3. About 20–100 kHz
  4. About 20–100 Hz
Answer and working

C. About 20–100 kHz

High-frequency, low-intensity ultrasound (MHz) is used for measurement.

Further reading: Sun, Emerging Technologies for Food Processing

5 · Ultrasound in processing and cleaning · easy

What is the main mechanism by which power ultrasound acts in liquids?

  1. Joule heating
  2. Acoustic cavitation
  3. Electroporation
  4. Ionising radiation
Answer and working

B. Acoustic cavitation

Bubble collapse produces hot spots, shock waves, shear, microjets and radicals.

Further reading: Sun, Emerging Technologies for Food Processing

6 · Supercritical fluid extraction · medium

Why is ethanol added as a co-solvent when extracting phospholipids?

  1. Ethanol sterilises the extraction vessel
  2. Supercritical CO2 is non-polar and dissolves them poorly
  3. Phospholipids react with CO2 to form gas
  4. Ethanol lowers the critical pressure of CO2 to zero
Answer and working

B. Supercritical CO2 is non-polar and dissolves them poorly

A polar modifier extends CO2's range to polar lipids.

Further reading: Brunner 2005

7 · Microencapsulation and nanotechnology · hard

How do regulators usually treat deliberately engineered nanomaterials in food?

  1. They treat them exactly as bulk materials
  2. They ban all nanostructures, including casein micelles
  3. They exempt them from any assessment
  4. They require specific safety assessment
Answer and working

D. They require specific safety assessment

Particle-specific behaviour requires characterisation and toxicology; natural dairy nanostructures are not treated as engineered.

Further reading: Sun, Emerging Technologies for Food Processing

8 · Regulatory approval of novel processes · medium

Under a pressure treatment, the target organism's D value is 0.8 min. What minimum holding time gives a 6-log reduction?

  1. 7.5 min
  2. 4.8 min
  3. 0.13 min
  4. 6.8 min
Answer and working

B. 4.8 min

6 × 0.8 = 4.8 min. 0.13 divides 0.8 by 6.

Further reading: Codex CXG 69-2008

9 · Regulatory approval of novel processes · medium

In EU novel food law, when is a food from a new process 'novel'?

  1. Only when the food is imported
  2. When the process significantly changes composition
  3. Whenever the factory or production line is newly built
  4. Whenever the process uses electricity at any stage
Answer and working

B. When the process significantly changes composition

Significant changes affecting nutrition, metabolism or undesirable substances trigger authorisation.

Further reading: Regulation (EU) 2015/2283

10 · Regulatory approval of novel processes · medium

Why can't pressure-treated milk simply be called 'pasteurised milk' in many jurisdictions?

  1. Pressure treatment is banned for all foods everywhere
  2. Pasteurisation is defined by thermal processes
  3. Pasteurised milk must be sold frozen
  4. Pressure-treated milk contains no protein
Answer and working

B. Pasteurisation is defined by thermal processes

Dairy standards list time–temperature combinations; other processes need specific recognition or a different name.

Further reading: Datta and Tomasula, Emerging Dairy Processing Technologies

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Read the subject

Emerging & Non-thermal Dairy ProcessingThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

More ICAR AICE-JRF/SRF practice

Rheology & Texture of Dairy FoodsFermentation Technology & Dairy BiotechnologyInstrumentation, Process Control & AutomationAdvanced Dairy MicrobiologyAdvanced Dairy Technology & Product DevelopmentAdvanced Dairy EngineeringResearch Methods, Experimental Design & Scientific CommunicationAdvanced Dairy ChemistryStarter Cultures & Lactic Acid BacteriaDairy Pathogens, Toxins & Microbial RiskQuality Assurance, Statistical Quality Control & Laboratory ManagementDairy Analysis & Instrumental MethodsMicrobiology of Raw & Market MilkChemistry of Dairy ProductsMilk Proteins & EnzymesMicrobiology of Dairy ProductsMembrane Processing & Dairy IngredientsLactose, Milk Salts & VitaminsProbiotics, Prebiotics & Functional Dairy FoodsMicrobiological Quality Assurance & Rapid MethodsDairy Effluent Treatment & Environmental ManagementMilk LipidsDairy Plant Utilities & Energy ManagementFermented Milks Technology

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