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ICAR AICE-JRF/SRF Chemistry of Dairy Products: practice questions

ICAR AICE-JRF/SRF (PhD): Dairy Chemistry, Dairy Microbiology, Dairy Technology, Dairy Engineering · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
87written to this exam in this subject
12syllabus units

What this subject covers

Physical and chemical changes in milk as it becomes cream, butter, ghee, concentrated and dried milks, cheese, fermented milks and frozen desserts.

In the exams: ICAR AICE subject 34 Units 5–8; ICAR AIEEA-PG group 16 (chemistry of cream, butter, ghee, ice cream, cheese, condensed and dried milks, infant food)

What ICAR AICE-JRF/SRF asks from it

ICAR AICE-JRF/SRF (PhD) — Dairy Chemistry (subject 34, Units 5-8)

Chemistry of cream, butter and ghee; heat stability, concentrated and dried milks, khoa, chhana and paneer; chemistry of cheese and fermented milks; ice cream. Questions test mechanisms such as churning, age gelation, rennet coagulation, ripening reactions, diacetyl formation and emulsifier action, and their links to defects.

Where the 87 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Chemistry of Dairy Products. Difficulty: 14 easy, 48 medium, 25 hard.

Changes during cheese ripening: glycolysis, proteolysis, lipolysis9
Chemistry of cream: neutralisation and ripening8
Milk powders: structure, solubility and instant properties8
Chemistry of rennet coagulation8
Ice cream: structure and role of stabilisers and emulsifiers8
Mechanism of churning and butter microstructure7
Concentrated milks: physico-chemical changes in manufacture and storage7
Chemistry of milk fermentation and flavour compounds7
Heat-desiccated and acid-coagulated products: khoa, chhana, paneer7
Heat stability of milk and the heat coagulation time6
Age gelation of UHT milk6
Ghee: composition by method, analytical constants, flavour and deterioration6

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Heat stability of milk and the heat coagulation time · medium

Above about pH 6.7, heating milk causes which change in kappa-casein that contributes to instability?

  1. Cleavage at Phe105-Met106 by heat alone without enzyme
  2. Conversion into alpha-s1-casein by isomerisation
  3. Dissociation from the micelles
  4. Crystallisation as calcium kappa-caseinate on the tube wall
Answer and working

C. Dissociation from the micelles

Kappa-casein (with denatured beta-lactoglobulin) leaves the micelles at higher pH, leaving micelles depleted of their stabilising layer.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

2 · Age gelation of UHT milk · medium

Which enzymes are chiefly responsible for proteolytic age gelation of UHT milk?

  1. Lipoprotein lipase and xanthine oxidoreductase
  2. Plasmin and heat-stable proteases of psychrotrophic bacteria
  3. Chymosin and pepsin carried over from cheese plants sharing the same raw milk
  4. Alkaline phosphatase and lactoperoxidase
Answer and working

B. Plasmin and heat-stable proteases of psychrotrophic bacteria

Plasmin and Pseudomonas proteases survive UHT; LPL, ALP and lactoperoxidase are inactivated and are not proteases.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

3 · Ghee: composition by method, analytical constants, flavour and deterioration · medium

What gives traditional ghee its granular texture?

  1. Slow cooling
  2. Rapid chilling to below 0 degrees Celsius
  3. Addition of sugar during clarification
  4. Residual casein forming lumps in the fat
Answer and working

A. Slow cooling

Slow cooling at moderate temperatures favours large crystal aggregates.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

4 · Chemistry of rennet coagulation · medium

Using Van Slyke's formula Y = (0.93F + C - 0.1) x 1.09 / (1 - W), what is the yield of Cheddar from 100 kilograms of milk with 3.6 per cent fat, 2.5 per cent casein and 37 per cent cheese moisture?

  1. About 9.9 kilograms
  2. About 9.1 kilograms
  3. About 6.3 kilograms
  4. About 15.8 kilograms
Answer and working

A. About 9.9 kilograms

(3.348 + 2.5 - 0.1) x 1.09/0.63 = 9.94 kg. 6.3 omits the moisture term; 9.1 omits the 1.09 factor.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

5 · Chemistry of rennet coagulation · medium

What does a high C/P ratio of a coagulant mean?

  1. High coagulation pH relative to ripening pH
  2. High casein content relative to protein
  3. High clotting activity relative to general proteolysis
  4. High calcium relative to phosphate
Answer and working

C. High clotting activity relative to general proteolysis

A high clotting-to-proteolysis ratio gives good yield and less bitterness.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

6 · Chemistry of rennet coagulation · easy

Which coagulant is now the most widely used in cheesemaking?

  1. Papain
  2. Calf rennet extracted from abomasa
  3. Pig pepsin from slaughterhouses
  4. Fermentation-produced chymosin
Answer and working

D. Fermentation-produced chymosin

Chymosin produced by microorganisms carrying the gene is widely used.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

7 · Chemistry of rennet coagulation · medium

Which coagulant is noted for very specific kappa-casein cleavage with little general proteolysis?

  1. Rhizomucor miehei protease
  2. Cryphonectria parasitica protease
  3. Camel chymosin
  4. Bovine pepsin
Answer and working

C. Camel chymosin

Camel chymosin has a high C/P ratio; mould proteases are more proteolytic.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

8 · Changes during cheese ripening: glycolysis, proteolysis, lipolysis · medium

Residual chymosin in cheese first hydrolyses which bond, producing alpha-s1-I-casein?

  1. Lys28-Lys29 of beta-casein
  2. Phe105-Met106 of kappa-casein
  3. Phe23-Phe24 of alpha-s1-casein
  4. Leu192-Tyr193 of beta-casein
Answer and working

C. Phe23-Phe24 of alpha-s1-casein

Chymosin cleaves alpha-s1-casein at Phe23-Phe24 early in ripening, softening texture. Lys28-Lys29 is a plasmin site.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

9 · Ice cream: structure and role of stabilisers and emulsifiers · easy

Which structural element of ice cream stabilises the air cells and gives melt resistance?

  1. Large lactose crystals
  2. Ice crystals above 100 micrometres
  3. Dissolved sucrose
  4. The partially coalesced fat network
Answer and working

D. The partially coalesced fat network

Partially coalesced fat surrounds air cells.

Further reading: Fox et al., Dairy Chemistry and Biochemistry

10 · Changes during cheese ripening: glycolysis, proteolysis, lipolysis · medium

Which agent in Dutch-type cheeses produces the small round openings?

  1. Clostridia producing hydrogen
  2. Moulds producing ammonia
  3. Citrate-fermenting lactococci and leuconostocs producing CO2
  4. Propionibacteria producing CO2 from lactate in the warm ripening room
Answer and working

C. Citrate-fermenting lactococci and leuconostocs producing CO2

Citrate fermentation gives CO2 and diacetyl, forming small eyes.

Further reading: Walstra, Wouters and Geurts, Dairy Science and Technology

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Read the subject

Chemistry of Dairy ProductsThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

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