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ICAR AICE-JRF/SRF Probiotics, Prebiotics & Functional Dairy Foods: practice questions

ICAR AICE-JRF/SRF (PhD): Dairy Chemistry, Dairy Microbiology, Dairy Technology, Dairy Engineering · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
80written to this exam in this subject
10syllabus units

What this subject covers

Probiotic organisms, prebiotics, bioactive components and the evidence and regulation behind health claims for dairy foods.

In the exams: ICAR AICE subject 36 Unit 5 and subject 37 Unit 6; ICAR AIEEA-PG group 16 (application of the probiotic concept)

What ICAR AICE-JRF/SRF asks from it

ICAR AICE-JRF/SRF (PhD) — Dairy Microbiology (subject 36, Unit 5) and Dairy Technology (subject 37, Unit 6)

Probiotic definitions and organisms, selection and safety criteria, mechanisms, survival and encapsulation, prebiotics and synbiotics, bioactive peptides, exopolysaccharides, functional food development, evidence and health claim regulation.

Where the 80 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Probiotics, Prebiotics & Functional Dairy Foods. Difficulty: 18 easy, 40 medium, 22 hard.

Probiotic genera: Lactobacillus (current genera), Bifidobacterium and others10
Survival in products and the gut; microencapsulation10
Prebiotics: galacto-oligosaccharides, inulin and human milk oligosaccharides9
Evidence standards for health benefits9
Regulation of probiotics and health claims worldwide8
Selection criteria and safety assessment of probiotic strains7
Mechanisms of probiotic action7
Bioactive peptides from milk proteins7
Exopolysaccharides from lactic acid bacteria7
Definitions: probiotics, prebiotics, synbiotics and postbiotics6

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Definitions: probiotics, prebiotics, synbiotics and postbiotics · medium

Why are postbiotics attractive to manufacturers?

  1. They can carry any health claim
  2. They need no characterisation
  3. They are always cheaper than milk
  4. No viability problem
Answer and working

D. No viability problem

Viability during storage is not a concern, though characterisation and evidence are still required.

Further reading: Tamime (ed.), Probiotic Dairy Products

2 · Probiotic genera: Lactobacillus (current genera), Bifidobacterium and others · easy

The spore-forming probiotic formerly called Bacillus coagulans is now named:

  1. Lactobacillus coagulans
  2. Clostridium coagulans
  3. Weissella coagulans
  4. Heyndrickxia coagulans
Answer and working

D. Heyndrickxia coagulans

Spores help survival in processed foods.

Further reading: Tamime (ed.), Probiotic Dairy Products

3 · Probiotic genera: Lactobacillus (current genera), Bifidobacterium and others · medium

Akkermansia muciniphila in pasteurised form has been authorised in the EU as:

  1. A novel food
  2. A starter culture
  3. A food colour
  4. A medicine for all patients
Answer and working

A. A novel food

Next-generation probiotics lack a history of safe use and need novel food or drug approval.

Further reading: Hill et al., ISAPP consensus statement on probiotics (2014)

4 · Mechanisms of probiotic action · medium

How do live yoghurt cultures help people with lactose maldigestion?

  1. They supply beta-galactosidase
  2. They convert lactose into fat
  3. They remove lactose before sale
  4. They produce lactase in the liver
Answer and working

A. They supply beta-galactosidase

The bacterial enzyme digests lactose in the gut.

Further reading: Hill et al., ISAPP consensus statement on probiotics (2014)

5 · Survival in products and the gut; microencapsulation · medium

Which standard describes counting bifidobacteria in milk products?

  1. ISO 22000
  2. ISO 6579
  3. ISO 29981 (IDF 220)
  4. ISO 4833
Answer and working

C. ISO 29981 (IDF 220)

TOS propionate agar with mupirocin, anaerobic incubation.

Further reading: Hill et al., ISAPP consensus statement on probiotics (2014)

6 · Prebiotics: galacto-oligosaccharides, inulin and human milk oligosaccharides · medium

How are 2'-FL and LNnT for infant formula produced commercially?

  1. By fermentation with engineered microorganisms
  2. By heating lactose with alkali
  3. By extraction from donated human milk in milk banks
  4. By hydrolysis of inulin
Answer and working

A. By fermentation with engineered microorganisms

Individual HMOs are made at scale by microbial fermentation.

Further reading: Hill et al., ISAPP consensus statement on probiotics (2014)

7 · Exopolysaccharides from lactic acid bacteria · medium

In lactococci, EPS genes are often located on:

  1. The cell wall
  2. Ribosomes
  3. Prophages only
  4. Plasmids
Answer and working

D. Plasmids

Plasmid loss can lead to loss of EPS production.

Further reading: Tamime (ed.), Probiotic Dairy Products

8 · Regulation of probiotics and health claims worldwide · medium

Why is the word probiotic generally not allowed on food labels in most EU member states?

  1. It is treated as an implied health claim
  2. It is a trademark
  3. It refers only to medicines
  4. It is considered offensive
Answer and working

A. It is treated as an implied health claim

Without an authorised claim, it cannot be used in most states.

Further reading: Hill et al., ISAPP consensus statement on probiotics (2014)

9 · Regulation of probiotics and health claims worldwide · easy

Japan's first regulatory system for functional foods, introduced in 1991, is:

  1. DSHEA
  2. QPS
  3. FOSHU
  4. PMO
Answer and working

C. FOSHU

Foods for Specified Health Uses are individually approved.

Further reading: Hill et al., ISAPP consensus statement on probiotics (2014)

10 · Regulation of probiotics and health claims worldwide · medium

Which labelling information is commonly expected for probiotic products?

  1. The colour of the culture
  2. The farm, region and breed of origin of the milk that was used
  3. Strain identity and viable count at end of shelf life
  4. Only the brand name
Answer and working

C. Strain identity and viable count at end of shelf life

Strain, count, storage and intake are standard expectations.

Further reading: Hill et al., ISAPP consensus statement on probiotics (2014)

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Read the subject

Probiotics, Prebiotics & Functional Dairy FoodsThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

More ICAR AICE-JRF/SRF practice

Rheology & Texture of Dairy FoodsFermentation Technology & Dairy BiotechnologyInstrumentation, Process Control & AutomationAdvanced Dairy MicrobiologyAdvanced Dairy Technology & Product DevelopmentAdvanced Dairy EngineeringResearch Methods, Experimental Design & Scientific CommunicationAdvanced Dairy ChemistryStarter Cultures & Lactic Acid BacteriaDairy Pathogens, Toxins & Microbial RiskQuality Assurance, Statistical Quality Control & Laboratory ManagementDairy Analysis & Instrumental MethodsMicrobiology of Raw & Market MilkChemistry of Dairy ProductsMilk Proteins & EnzymesMicrobiology of Dairy ProductsMembrane Processing & Dairy IngredientsLactose, Milk Salts & VitaminsMicrobiological Quality Assurance & Rapid MethodsDairy Effluent Treatment & Environmental ManagementMilk LipidsEmerging & Non-thermal Dairy ProcessingDairy Plant Utilities & Energy ManagementFermented Milks Technology

Probiotics, Prebiotics & Functional Dairy Foods for other exams

ICAR AIEEA-PG · IndiaASRB NET and ARS · India

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