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ICAR AICE-JRF/SRF Fermented Milks Technology: practice questions

ICAR AICE-JRF/SRF (PhD): Dairy Chemistry, Dairy Microbiology, Dairy Technology, Dairy Engineering · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
69written to this exam in this subject
11syllabus units

What this subject covers

Yoghurt, dahi and the world's fermented milks: process, cultures, texture, defects and shelf life.

In the exams: ICAR AICE subjects 34 Unit 7 and 36 Unit 5; ICAR AIEEA-PG group 16 (dahi, yoghurt, kefir, koumiss, Bulgarian milk, cultured buttermilk, leben, yakult); GATE XL-U Section 2 (curd, yoghurt)

What ICAR AICE-JRF/SRF asks from it

ICAR AICE-JRF/SRF (PhD) — Dairy Chemistry (34) and Dairy Microbiology (36)

Subject 34 Unit 7: theories and pathways of fermentation, composition and chemical changes in dahi, yoghurt and acidophilus milk, flavour development, lassi and buttermilk, nutritional and therapeutic significance. Subject 36 Unit 5: starter cultures of lactic, yeast-lactic and mould-lactic fermented milks, spoilage and safety, probiotics and their regulation.

Where the 69 questions fall

How our questions for ICAR AICE-JRF/SRF spread across the units of Fermented Milks Technology. Difficulty: 3 easy, 43 medium, 23 hard.

Probiotic fermented milks10
Yoghurt: set, stirred, drinking and strained types9
Skyr, labneh and other concentrated fermented milks8
Process steps and their effect on texture7
Kefir, koumiss and yeast-lactic fermented milks7
Syneresis, post-acidification and other defects7
Classification of fermented milks worldwide5
Fruit preparations and flavouring5
Shelf life of fermented milks5
Cultured buttermilk and sour cream4
Dahi, misti dahi, shrikhand and lassi2

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Classification of fermented milks worldwide · medium

Which fermented milk is classed as yeast-lactic?

  1. Acidophilus milk
  2. Kefir
  3. Cultured buttermilk
  4. Yoghurt
Answer and working

B. Kefir

Kefir contains lactic acid bacteria and yeasts. Yoghurt, cultured buttermilk and acidophilus milk are lactic fermentations only.

Further reading: Tamime (ed.), Fermented Milks

2 · Classification of fermented milks worldwide · medium

Under the Codex standard, what applies to a fermented milk that is heat-treated after fermentation?

  1. It must be re-inoculated with live culture before it is sold
  2. The requirement for viable starter organisms does not apply
  3. It may no longer be described as a milk product of any kind
  4. It must still contain at least 10^7 cfu/g of live starter
Answer and working

B. The requirement for viable starter organisms does not apply

Codex exempts heat-treated-after-fermentation products from the viable-culture requirement, and labelling must reflect the treatment where national law requires. They remain dairy products.

Further reading: Tamime (ed.), Fermented Milks

3 · Process steps and their effect on texture · hard

Mechanistically, why does heat-denatured beta-lactoglobulin improve yoghurt texture?

  1. It binds kappa-casein, adding strands and water-binding sites to the gel
  2. It hydrolyses kappa-casein so that the milk clots as in cheese making
  3. It converts lactose to galactose, which raises viscosity in the gel
  4. It chelates calcium so that colloidal calcium phosphate stays intact
Answer and working

A. It binds kappa-casein, adding strands and water-binding sites to the gel

Denatured beta-lactoglobulin complexes with kappa-casein via disulphide interchange; as pH falls, these complexes take part in aggregation, giving more cross-links and a firmer, less syneretic gel.

Further reading: Tamime and Robinson, Yoghurt: Science and Technology

4 · Process steps and their effect on texture · medium

Why are milk powders deaerated or given hydration time before yoghurt making?

  1. Powder must ferment for some hours before inoculation
  2. Air is needed for culture growth and must be added
  3. Hydration time is needed to denature the whey proteins
  4. Undissolved powder and air cause grainy texture
Answer and working

D. Undissolved powder and air cause grainy texture

Poorly dissolved powder gives graininess and air causes foaming and surface defects. Yoghurt cultures do not need air.

Further reading: Tamime and Robinson, Yoghurt: Science and Technology

5 · Dahi, misti dahi, shrikhand and lassi · medium

Buffalo milk dahi is typically firmer than cow milk dahi because buffalo milk has:

  1. Higher casein, fat and total solids
  2. More lactose but much less protein overall
  3. Lower casein and fat
  4. No whey proteins at all
Answer and working

A. Higher casein, fat and total solids

Higher solids, especially casein, give a firmer acid gel.

Further reading: Tamime and Robinson, Yoghurt: Science and Technology

6 · Kefir, koumiss and yeast-lactic fermented milks · medium

Packaged kefir is reported to swell its packs during retail storage. The most likely cause is:

  1. Mould growth on the inside of the lid
  2. Coliform contamination from the plant's water supply
  3. Continuing yeast activity producing carbon dioxide
  4. Post-acidification by Lactobacillus bulgaricus
Answer and working

C. Continuing yeast activity producing carbon dioxide

Kefir yeasts keep producing gas in the pack; culture choice, temperature and pack design control it.

Further reading: Tamime (ed.), Fermented Milks

7 · Cultured buttermilk and sour cream · hard

Which organisms in a buttermilk culture convert citrate to diacetyl?

  1. L. lactis biovar diacetylactis and Leuconostoc mesenteroides subsp. cremoris
  2. Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis
  3. Kluyveromyces marxianus and Saccharomyces cerevisiae
  4. Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus
Answer and working

A. L. lactis biovar diacetylactis and Leuconostoc mesenteroides subsp. cremoris

The citrate-positive lactococcus and leuconostoc are the aroma producers in mesophilic cultures.

Further reading: Tamime (ed.), Fermented Milks

8 · Skyr, labneh and other concentrated fermented milks · medium

Which method concentrates fermented curd continuously by centrifugal force?

  1. A nozzle separator of the quark type
  2. A plate heat exchanger
  3. A homogeniser run at very high pressure
  4. A cloth bag hung overnight
Answer and working

A. A nozzle separator of the quark type

Nozzle separators concentrate acid curd continuously; cloth straining is slow and batch; heat exchangers and homogenisers do not separate whey.

Further reading: Tamime (ed.), Fermented Milks

9 · Probiotic fermented milks · hard

Why are cultures without L. bulgaricus (such as acidophilus–bifidus–thermophilus cultures) used for some probiotic yoghurts?

  1. L. bulgaricus raises the pH and lets spoilage bacteria grow
  2. L. bulgaricus is a pathogen and is banned from probiotic products
  3. L. bulgaricus cannot grow in milk without added probiotics
  4. L. bulgaricus post-acidification harms probiotic survival
Answer and working

D. L. bulgaricus post-acidification harms probiotic survival

Its continued acid production in the cold harms acid-sensitive probiotics.

Further reading: Tamime and Robinson, Yoghurt: Science and Technology

10 · Fruit preparations and flavouring · medium

Why must fruit preparations for yoghurt be heat-treated and filled aseptically?

  1. They get no further heat, so contaminants would spoil the yoghurt
  2. Heat treatment converts fruit sugars into lactic acid before use
  3. Heat treatment is needed to activate the yoghurt culture in the fruit
  4. Aseptic filling is needed to keep the fruit pieces from sinking
Answer and working

A. They get no further heat, so contaminants would spoil the yoghurt

Yeasts and moulds in fruit preparations are a classic cause of blown lids; the yoghurt is not heated after fruit addition.

Further reading: Tamime (ed.), Fermented Milks

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Read the subject

Fermented Milks TechnologyThe full study manual, free ICAR AICE-JRF/SRFFormat, sections and how it has changed

More ICAR AICE-JRF/SRF practice

Rheology & Texture of Dairy FoodsFermentation Technology & Dairy BiotechnologyInstrumentation, Process Control & AutomationAdvanced Dairy MicrobiologyAdvanced Dairy Technology & Product DevelopmentAdvanced Dairy EngineeringResearch Methods, Experimental Design & Scientific CommunicationAdvanced Dairy ChemistryStarter Cultures & Lactic Acid BacteriaDairy Pathogens, Toxins & Microbial RiskQuality Assurance, Statistical Quality Control & Laboratory ManagementDairy Analysis & Instrumental MethodsMicrobiology of Raw & Market MilkChemistry of Dairy ProductsMilk Proteins & EnzymesMicrobiology of Dairy ProductsMembrane Processing & Dairy IngredientsLactose, Milk Salts & VitaminsProbiotics, Prebiotics & Functional Dairy FoodsMicrobiological Quality Assurance & Rapid MethodsDairy Effluent Treatment & Environmental ManagementMilk LipidsEmerging & Non-thermal Dairy ProcessingDairy Plant Utilities & Energy Management

Fermented Milks Technology for other exams

ICAR AIEEA-PG · India

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