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ICAR AIEEA Veterinary Clinical Biochemistry & Laboratory Diagnosis: practice questions

ICAR AIEEA (PG): Master's entrance, Veterinary Science and Animal Sciences · India · NTA for ICAR

Original practice questions written to the published syllabus · not past papers
10worked here, free
20written to this exam in this subject
8syllabus units

About ICAR AIEEA

ICAR AIEEA (PG): Master's entrance, Veterinary Science and Animal Sciences is a postgraduate admission exam run by NTA for ICAR, for candidates in India. Computer-based, 120 MCQs in 120 minutes, +4 for correct and -1 for wrong, maximum 480. English only.

Source: icar.org.in.

Checked against the exam body's published information. Formats and fees change; confirm the current edition there before you book.

Veterinary Clinical Biochemistry & Laboratory Diagnosis in ICAR AIEEA

Red cell indices and their calculation, anaemia classification, liver and kidney function tests, urinalysis, anticoagulants, acute phase proteins, enzyme specificity by species, and quality control concepts.

Using the laboratory well: haematology, biochemistry panels, organ function tests, urinalysis, cytology, sampling for microbiology, reference intervals and quality control.

Our 20 ICAR AIEEA questions in this subject: 16 easy, 4 medium, 0 hard, across 8 units.

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

These 10 were written to ICAR AIEEA and are not shown on any other exam's page.

Question 1 · easy

Which anticoagulant is preferred for a routine mammalian complete blood count and blood smear?

  1. Sodium citrate, because it is reversible for later clotting work
  2. Sodium fluoride, because it stops cells from metabolising glucose
  3. Lithium heparin, because it gives the best-stained leukocytes
  4. EDTA, because it preserves cell morphology best
Answer and working

D. EDTA, because it preserves cell morphology best

EDTA chelates calcium and preserves cell morphology, so it is used for the CBC and smear. Heparin is the common wrong choice: it causes clumping and poor staining of leukocytes in mammals, although it is used for many birds and reptiles whose red cells EDTA damages. Citrate is for coagulation and fluoride for glucose.

Question 2 · medium

A piglet aged three weeks, housed indoors without iron supplementation, is pale. Which red cell picture is expected?

  1. Microcytic, hypochromic anaemia
  2. Macrocytic, hypochromic anaemia
  3. Normocytic, normochromic anaemia
  4. Macrocytic, normochromic anaemia
Answer and working

A. Microcytic, hypochromic anaemia

Iron deficiency impairs haemoglobin synthesis, so extra divisions produce small (microcytic) cells with less haemoglobin (hypochromic). Piglets are born with low iron stores and sow's milk is iron-poor. Macrocytic hypochromic is the pattern of strong regeneration, and normocytic normochromic is typical of anaemia of inflammation.

Question 3 · easy

Which statement about the normal leukogram of adult cattle is correct?

  1. Neutrophils normally outnumber lymphocytes about three to one
  2. Lymphocytes normally outnumber neutrophils
  3. Eosinophils are normally absent from healthy bovine blood
  4. Band neutrophils normally exceed segmented neutrophils
Answer and working

B. Lymphocytes normally outnumber neutrophils

Adult cattle are lymphocyte-predominant, with a neutrophil to lymphocyte ratio around 1:2; a reversal suggests inflammation or stress. Neutrophil predominance is the pattern of dogs, cats and horses. Eosinophils are normally present, and bands should be few.

Question 4 · medium

A young male dog bleeds after castration. Platelet count and PT are normal; aPTT is prolonged. Which defect fits best?

  1. Von Willebrand disease with normal factor activity
  2. Haemophilia A (factor VIII deficiency)
  3. Anticoagulant rodenticide toxicosis (vitamin K antagonism)
  4. Immune-mediated thrombocytopenia
Answer and working

B. Haemophilia A (factor VIII deficiency)

Factor VIII belongs to the intrinsic pathway, so its deficiency prolongs aPTT while PT stays normal; haemophilia A is X-linked and seen in males. Von Willebrand disease prolongs bleeding time with usually normal PT; rodenticide prolongs PT first; ITP lowers the platelet count.

Question 5 · medium

A dog has hypoalbuminaemia (18 g/L) and low total calcium but normal ionised calcium. What is the best interpretation?

  1. Total calcium is low because less is protein-bound; no treatment for calcium
  2. True hypocalcaemia needing intravenous calcium gluconate immediately
  3. Hypoparathyroidism needing vitamin D therapy for the rest of life
  4. Puerperal tetany, which occurs in bitches regardless of lactation
Answer and working

A. Total calcium is low because less is protein-bound; no treatment for calcium

About half of total calcium is protein-bound, mostly to albumin. Hypoalbuminaemia lowers total calcium while the active ionised fraction is normal. Measure ionised calcium rather than relying on correction formulas.

Question 6 · easy

Grass tetany in grazing cattle is associated with a low blood concentration of which mineral?

  1. Phosphorus
  2. Copper
  3. Magnesium
  4. Sodium
Answer and working

C. Magnesium

Grass tetany (hypomagnesaemic tetany) occurs on lush, potassium-rich pasture with low magnesium intake or absorption, often with concurrent hypocalcaemia. Low phosphorus is linked to post-parturient haemoglobinuria; copper deficiency causes anaemia, poor growth and demyelination.

Question 7 · easy

Which enzyme is liver-specific in horses and ruminants but unstable in stored samples?

  1. Sorbitol dehydrogenase
  2. Glutamate dehydrogenase
  3. Aspartate aminotransferase
  4. Alkaline phosphatase
Answer and working

A. Sorbitol dehydrogenase

SDH is liver-specific in large animals but loses activity within hours, so it must be measured promptly. GLDH is also liver-specific and more stable. AST also comes from muscle; ALP is not liver-specific and very variable in large animals.

Question 8 · easy

What is the expected pH of urine from a healthy adult herbivore such as a horse or cow?

  1. Strongly acidic
  2. Neutral and fixed
  3. Alkaline
  4. Acidic after every meal
Answer and working

C. Alkaline

Plant diets yield an alkaline urine in herbivores; carnivores usually have acidic urine. Aciduria in a cow suggests metabolic disturbance such as paradoxical aciduria or an acidifying diet.

Question 9 · easy

Why should tissue for PCR not be placed in formalin?

  1. Formalin amplifies DNA and so gives false-positive PCR results
  2. Formalin is required by every laboratory before PCR testing
  3. Formalin degrades nucleic acid and reduces PCR sensitivity
  4. Formalin makes all viruses in the tissue grow in cell culture
Answer and working

C. Formalin degrades nucleic acid and reduces PCR sensitivity

Formalin cross-links and fragments nucleic acids, lowering PCR yield. Fresh, chilled or frozen tissue, or a dedicated nucleic acid preservative, is preferred.

Question 10 · easy

Which in-clinic test gives the PCV, buffy coat, plasma colour and plasma for total protein in a few minutes?

  1. Lateral-flow immunoassay of whole blood
  2. Microhaematocrit centrifugation
  3. Dipstick urinalysis on a voided sample
  4. Blood gas analysis on an arterial sample
Answer and working

B. Microhaematocrit centrifugation

Spinning a microhaematocrit tube gives the PCV, buffy coat height, plasma colour (icterus, haemolysis, lipaemia) and plasma for refractometric total protein.

A study plan for Veterinary Clinical Biochemistry & Laboratory Diagnosis in ICAR AIEEA

This plan follows where our questions actually fall, so the time goes where the marks are likely to be. It is our reading of the syllabus, not the exam body's weighting.

  1. First, the heavy units (70% of the 20 questions): Haematology (6, 30%); Serum biochemistry panels (6, 30%); Kidney function tests (2, 10%).

    Read the manual chapters for these first and do every worked question you can find on them.

  2. Then the middle band: Liver function tests (2, 10%); Cytology (1, 5%); Point-of-care testing (1, 5%).

    Aim for one sitting per unit, then mixed questions across them.

  3. Then the lighter units: Sampling for microbiology (1, 5%); Urinalysis (1, 5%).

    Learn the definitions, classifications and standard numbers; these units are more often recalled than worked.

  4. Bank the easy marks: Kidney function tests (2 easy of 2); Liver function tests (2 easy of 2).

    Do these early in revision so they are fast on the day.

  5. Finish timed. Sit the subject as a timed set without notes, mark it, and send each wrong answer back to its unit above.

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