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National Licensure Examination for Veterinarians Environment & Environmental Hygiene: practice questions

National Licensure Examination for Veterinarians (NLEV) · Nepal · Nepal Veterinary Council (NVC Act 2055 BS)

Original practice questions written to the published syllabus · not past papers
10worked here, free
50written to this exam in this subject
9syllabus units

What this subject covers

Water and air hygiene, waste and carcass disposal, disinfection, pollution and climate change as they affect animal and human health.

In the exams: NAVLE environmental health and safety 5%

Where the 50 questions fall

How our questions for National Licensure Examination for Veterinarians spread across the units of Environment & Environmental Hygiene. Difficulty: 19 easy, 26 medium, 5 hard.

Water hygiene10
Waste management on livestock farms9
Disinfection8
Air hygiene6
Environmental pollution6
Biomedical waste5
Climate change and livestock4
Carcass disposal1
Experimental animal medicine1

10 practice questions, worked

Choose an answer, then open the working. Each explanation says why the right answer is right and why the others are not.

1 · Water hygiene · easy

The result of the multiple-tube fermentation test for coliforms is expressed as:

  1. Plaque-forming units per millilitre of sample
  2. Colony-forming units per gram of sediment
  3. Most probable number per 100 mL
  4. Optical density at 600 nm of the broth
Answer and working

C. Most probable number per 100 mL

Tube results are converted with statistical tables to a most probable number per 100 mL. Colony counts per 100 mL come from membrane filtration, not from tubes; plaque counts are for phages and viruses.

Further reading: APHA Standard Methods for the Examination of Water and Wastewater

2 · Water hygiene · medium

In membrane filtration testing of water, the pore size of the filter normally used to retain coliform bacteria is:

  1. 0.02 µm
  2. 45 µm
  3. 0.45 µm
  4. 4.5 µm
Answer and working

C. 0.45 µm

A 0.45 µm membrane retains coliforms while allowing water through at a practical rate; colonies on the filter are counted directly per 100 mL. 4.5 µm and 45 µm would let bacteria pass; 0.02 µm is an ultrafiltration size that would clog quickly.

Further reading: APHA Standard Methods

3 · Water hygiene · medium

Why is chlorination more effective in water at pH 6.5 than at pH 8.5?

  1. Chloramines form faster and they kill bacteria best
  2. More of the free chlorine is present as hypochlorite ion
  3. Chlorine gas is less soluble in acid water
  4. More of the free chlorine is present as hypochlorous acid
Answer and working

D. More of the free chlorine is present as hypochlorous acid

Free chlorine exists as hypochlorous acid (HOCl) and hypochlorite ion (OCl−); HOCl dominates at lower pH and is the far more effective biocide because it is uncharged and penetrates cells. Hypochlorite ion is the weaker form that dominates at high pH. Chloramines are slower, weaker disinfectants.

Further reading: WHO Guidelines for Drinking-water Quality; McDonnell, Antisepsis, Disinfection and Sterilization

4 · Waste management on livestock farms · hard

In a BOD test, 6 mL of dairy effluent is diluted to 300 mL. Dissolved oxygen is 8.6 mg/L at the start and 3.4 mg/L after 5 days at 20 °C. What is the BOD5 of the effluent?

  1. 255 mg/L
  2. 52 mg/L
  3. 260 mg/L
  4. 5.2 mg/L
Answer and working

C. 260 mg/L

BOD = (DO initial − DO final) / P, where P = 6/300 = 0.02. BOD = (8.6 − 3.4) / 0.02 = 5.2 / 0.02 = 260 mg/L. 5.2 forgets the dilution; 255 uses 6/294 (sample to diluent) instead of sample to total volume; 52 uses a factor of 10.

Further reading: APHA Standard Methods

5 · Waste management on livestock farms · medium

For efficient aerobic composting of manure, the starting carbon to nitrogen ratio should be about:

  1. 1 : 1
  2. 25–30 : 1
  3. 100 : 1
  4. 5 : 1
Answer and working

B. 25–30 : 1

A C:N of about 25–30:1 balances microbial energy (carbon) and protein (nitrogen) needs. Ratios as low as 5:1 lose nitrogen as ammonia; at 100:1 composting is slow because nitrogen limits microbial growth.

Further reading: On-farm composting handbooks (NRAES-54)

6 · Waste management on livestock farms · easy

Biogas from anaerobic digestion of cattle dung consists mainly of:

  1. Hydrogen, about 80%, with the rest nitrogen
  2. Methane, about 50–70%, with most of the rest carbon dioxide
  3. Carbon monoxide, about 60%, with the rest methane
  4. Carbon dioxide, about 90%, with traces of methane
Answer and working

B. Methane, about 50–70%, with most of the rest carbon dioxide

Biogas is typically 50–70% methane and 30–50% carbon dioxide, with traces of hydrogen sulphide and water vapour. Carbon monoxide is not a normal digestion product.

Further reading: FAO and national biogas manuals

7 · Carcass disposal · easy

Why should carcasses be moved as little as possible during an outbreak of a contagious disease?

  1. Moving them speeds decomposition so they cannot be sampled any more
  2. Moving them is illegal in every country whatever the disease involved
  3. Transport spreads the pathogen along the route and to other premises
  4. Moving them destroys the pathogen so samples taken later are negative
Answer and working

C. Transport spreads the pathogen along the route and to other premises

Leaking fluids, flies and contaminated vehicles can spread infection along the route and at the destination; when carcasses must be moved, leak-proof covered vehicles and cleaned routes are used. Transport is restricted, not universally banned.

Further reading: WOAH Terrestrial Code, disposal of dead animals

8 · Biomedical waste · easy

Under India's Bio-Medical Waste Management Rules, 2016, used hypodermic needles from a veterinary hospital go into:

  1. A blue cardboard box
  2. White translucent puncture-proof container
  3. A red non-chlorinated plastic bag with a biohazard label
  4. A yellow non-chlorinated plastic bag
Answer and working

B. White translucent puncture-proof container

Sharps, including needles and blades, go into white translucent puncture-proof containers. Red bags take contaminated recyclable plastics such as tubing and syringes without needles; yellow takes anatomical and soiled waste; blue takes glassware and metal implants.

Further reading: Bio-Medical Waste Management Rules, 2016 (India)

9 · Biomedical waste · easy

Under India's Bio-Medical Waste Management Rules, 2016, placental tissue from a caesarean section in a cow goes into:

  1. A yellow bag, for incineration or approved alternative
  2. A red bag, for autoclaving, shredding and then recycling
  3. A blue box, for disinfection and recycling
  4. A black bag, as general municipal waste
Answer and working

A. A yellow bag, for incineration or approved alternative

Anatomical waste, including tissues, organs and placentas, is yellow-category waste sent for incineration, plasma pyrolysis or permitted deep burial. Red is for recyclable contaminated plastics and blue for glass; anatomical waste is never general waste.

Further reading: Bio-Medical Waste Management Rules, 2016 (India)

10 · Disinfection · easy

Why is 70% ethanol a better disinfectant than absolute (100%) ethanol?

  1. 70% ethanol is more acidic, so it kills spores
  2. 100% ethanol reacts with water to form an inactive salt
  3. Water is needed for effective protein denaturation and penetration
  4. 100% ethanol is too volatile to wet the surface at all
Answer and working

C. Water is needed for effective protein denaturation and penetration

Water assists protein denaturation and allows alcohol to penetrate cells; absolute alcohol dehydrates and coagulates surface proteins, which protects the organism. Neither strength kills bacterial spores.

Further reading: McDonnell, Antisepsis, Disinfection and Sterilization

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Read the subject

Environment & Environmental HygieneThe full study manual, free National Licensure Examination for VeterinariansFormat, sections and how it has changed

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Environment & Environmental Hygiene for other exams

ENARE multiprofessional residency · BrazilICAR AIEEA · IndiaRCVS Statutory Membership Examination · UKASRB NET and ARS / SMS / STO · IndiaICAR AICE-JRF/SRF · IndiaNAVLE · USA / CanadaBCSE · USA / CanadaAustralasian Veterinary Examination, formerly the National Veterinary Examination · Australia / New ZealandENADE · BrazilNational Veterinary Examination · JapanRPSC Veterinary Officer · IndiaTNPSC Animal Husbandry & Veterinary Science, Code 396 · India

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